##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AspI AvrII BamHI BbrPI BcgI
BfrI BlnI BsaBI BsiWI BspDI
BspHI BssHII BstXI Bsu36I ClaI
CpoI DrdI Eco47III Eco81I FseI
HindIII HpaI KspI MamI MluI
NcoI NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SgrAI SnaBI SphI Sse8387I
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflIII 4646 ACATGT A^C[GA][CT]GT
AhdI 302 GACCATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 302 GACCATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 173 GTGCTC G[AT]GC[AT]^C
AviII 3522 TGCGCA TGC^GCA
BalI 3751 TGGCCA TGG^CCA
BclI 2651 TGATCA T^GATCA
BglI 227 GCCTGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2604 AGATCT A^GATCT
BlpI 3580 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3580 GCTCAGC GC^T[ACGT]AGC
BsaAI 4931 TACGTG [CT]AC^GT[GA]
BsiHKAI 173 GTGCTC G[AT]GC[AT]^C
BsmBI 2459 CGTCTC CGTCTC(1/5)
BsmI 3352 GCATTC GAATGC(1/-1)
Bsp1407I 2502 TGTACA T^GTACA
BspLU11I 4646 ACATGT A^CATGT
BsrGI 2502 TGTACA T^GTACA
BssSI 714 CTCGTG CACGAG(-5/-1)
Bst1107I 4928 GTATAC GTA^TAC
BstBI 1578 TTCGAA TT^CGAA
BstEII 83 GGTAACC G^GT[ACGT]ACC
BstPI 83 GGTAACC G^GT[ACGT]ACC
BstZ17I 4928 GTATAC GTA^TAC
CelII 3580 GCTCAGC GC^T[ACGT]AGC
Eam1105I 302 GACCATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 83 GGTAACC G^GT[ACGT]ACC
EcoRV 1239 GATATC GAT^ATC
EcoT22I 4098 ATGCAT ATGCA^T
FbaI 2651 TGATCA T^GATCA
FspI 3522 TGCGCA TGC^GCA
HincII 1628 GTCAAC GT[CT]^[GA]AC
HindII 1628 GTCAAC GT[CT]^[GA]AC
MfeI 908 CAATTG C^AATTG
MluNI 3751 TGGCCA TGG^CCA
MscI 3751 TGGCCA TGG^CCA
MslI 2538 CACGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 908 CAATTG C^AATTG
NdeI 5044 CATATG CA^TATG
NsiI 4098 ATGCAT ATGCA^T
NspV 1578 TTCGAA TT^CGAA
PshAI 3170 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 1578 TTCGAA TT^CGAA
SpeI 1083 ACTAGT A^CTAGT
SspBI 2502 TGTACA T^GTACA
XbaI 4735 TCTAGA T^CTAGA
XcmI 2426 CCACTCCAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 1500 GGTACC G^GTACC
2596 GGTACC
AccI 4263 GTAGAC GT^[AC][GT]AC
4928 GTATAC
AccIII 125 TCCGGA T^CCGGA
4765 TCCGGA
AlwI 4590 GGATC GGATC(4/5)
4732 GGATC
Asp700I 1847 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4672 GAATGAATTC
Asp718I 1500 GGTACC G^GTACC
2596 GGTACC
BbeI 374 GGCGCC GGCGC^C
3264 GGCGCC
BsaWI 125 TCCGGA [AT]^CCGG[AT]
4765 TCCGGA
BseAI 125 TCCGGA T^CCGGA
4765 TCCGGA
BspEI 125 TCCGGA T^CCGGA
4765 TCCGGA
BstAPI 1462 GCAACTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1471 GCACCAAGTGC
DraIII 1472 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2216 CACTTGGTG
EcoNI 136 CCTATGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1972 CCTACCCCAGG
EcoT14I 2154 CCAAGG C^C[AT][AT]GG
4091 CCTTGG
HgaI 97 GCGTC GACGC(5/10)
308 GCGTC
KasI 374 GGCGCC G^GCGCC
3264 GGCGCC
KpnI 1500 GGTACC GGTAC^C
2596 GGTACC
MroI 125 TCCGGA T^CCGGA
4765 TCCGGA
NarI 374 GGCGCC GG^CGCC
3264 GGCGCC
ScaI 2833 AGTACT AGT^ACT
4135 AGTACT
SfoI 374 GGCGCC GGC^GCC
3264 GGCGCC
StyI 2154 CCAAGG C^C[AT][AT]GG
4091 CCTTGG
XmnI 1847 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4672 GAATGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwNI ApoI AseI AsnI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI StuI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI