##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
Alw44I AlwNI AocI ApaI ApaLI
AscI Asp718I AspHI AspI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BciVI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaBI BseAI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII EagI EclXI Eco52I
Eco81I EcoO109I EcoO65I FauI FseI
FspI HgaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MspA1I MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoII
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AciI 66 CCGC CCGC(-3/-1)
AflII 995 CTTAAG C^TTAAG
AflIII 3210 ACATGT A^C[GA][CT]GT
AlwI 729 GGATC GGATC(4/5)
Aor51HI 3538 AGCGCT AGC^GCT
BbvI 1888 GCAGC GCAGC(8/12)
BcgI 84 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 995 CTTAAG C^TTAAG
BsaAI 217 CACGTA [CT]AC^GT[GA]
BsaI 440 GGTCTC GGTCTC(1/5)
BsaWI 2193 ACCGGA [AT]^CCGG[AT]
BsmI 3264 GAATGC GAATGC(1/-1)
BspLU11I 3210 ACATGT A^CATGT
BstXI 2874 CCAGTGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 3411 GACATTAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3538 AGCGCT AGC^GCT
EcoNI 2897 CCTTTAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3266 ATGCAT ATGCA^T
Fnu4HI 1888 GCAGC GC^[ACGT]GC
HaeII 3538 AGCGCT [GA]GCGC^[CT]
HpaI 1994 GTTAAC GTT^AAC
ItaI 1888 GCAGC GC^[ACGT]GC
NdeI 3373 CATATG CA^TATG
NsiI 3266 ATGCAT ATGCA^T
PaeR7I 104 CTCGAG C^TCGAG
PflMI 3851 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 436 ACCTGGT A^CC[AT]GGT
SphI 1080 GCATGC GCATG^C
SspI 3718 AATATT AAT^ATT
TseI 1888 GCAGC G^C[AT]GC
Van91I 3851 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3973 CCAGTAGTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 104 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Asp700I 473 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
887 GAATAATTTC
AvaII 383 GGACC G^G[AT]CC
3369 GGTCC
BbvCI 95 CCTCAGC CCTCAGC(-5/-2)
206 CCTCAGC
BclI 686 TGATCA T^GATCA
3775 TGATCA
BseRI 287 GAGGAG GAGGAG(10/8)
366 GAGGAG
BsgI 144 GTGCAG GTGCAG(16/14)
1886 GTGCAG
BsmFI 409 GGGAC GGGAC(10/14)
522 GGGAC
BspMI 1920 ACCTGC ACCTGC(4/8)
2587 GCAGGT
BstAPI 2578 GCACACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2836 GCACAACATGC
Cac8I 1051 GCAAGC GC[ACGT]^[ACGT]GC
1080 GCATGC
CfoI 492 GCGC GCG^C
3539 GCGC
DsaI 881 CCATGG C^C[GA][CT]GG
2937 CCATGG
EcoRV 450 GATATC GAT^ATC
3668 GATATC
FbaI 686 TGATCA T^GATCA
3775 TGATCA
HhaI 492 GCGC GCG^C
3539 GCGC
HinP1I 492 GCGC G^CGC
3539 GCGC
NcoI 881 CCATGG C^CATGG
2937 CCATGG
NlaIV 383 GGACCC GG[ACGT]^[ACGT]CC
433 GGAACC
SmaI 72 CCCGGG CCC^GGG
132 CCCGGG
TaqI 105 TCGA T^CGA
1343 TCGA
TthHB8I 105 TCGA T^CGA
1343 TCGA
XmaCI 72 CCCGGG C^CCGGG
132 CCCGGG
XmaI 72 CCCGGG C^CCGGG
132 CCCGGG
XmnI 473 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
887 GAATAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AatII AccI AcsI AcyI AfaI
AhdI AluI ApoI AseI AsnI
AspEI AvaI BalI BbsI BcnI
BfaI BpmI BpuAI BsaHI BsaJI
BsiYI BslI BsmAI BsoBI BspHI
BsrDI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraI EaeI
Eam1105I EarI EcoRI EcoRII EcoT14I
FokI HaeIII HapII Hin1I HincII
HindII HindIII HinfI HpaII HphI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NspI PleI PshBI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TfiI Tru9I
Tsp45I Tsp509I TspRI