##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
AseI AsnI Asp700I Asp718I BglII
BsiWI Bsp1407I BspDI BsrGI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI Eco47III EcoRV FseI HincII
HindII HindIII HpaI KpnI MluI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI ScaI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 521 GACGTC GACGT^C
AccIII 3937 TCCGGA T^CCGGA
AfaI 5878 GTAC GT^AC
AflII 6945 CTTAAG C^TTAAG
AspI 2304 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 463 TGCGCA TGC^GCA
AvrII 5169 CCTAGG C^CTAGG
BamHI 2935 GGATCC G^GATCC
BclI 544 TGATCA T^GATCA
BfrI 6945 CTTAAG C^TTAAG
BlnI 5169 CCTAGG C^CTAGG
BsaBI 2184 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3937 TCCGGA [AT]^CCGG[AT]
BseAI 3937 TCCGGA T^CCGGA
BsmI 4778 GCATTC GAATGC(1/-1)
BspEI 3937 TCCGGA T^CCGGA
BspHI 5317 TCATGA T^CATGA
BspLU11I 6190 ACATGT A^CATGT
BssHII 2578 GCGCGC G^CGCGC
DraI 595 TTTAAA TTT^AAA
EcoRI 7148 GAATTC G^AATTC
EcoT22I 4375 ATGCAT ATGCA^T
FbaI 544 TGATCA T^GATCA
FspI 463 TGCGCA TGC^GCA
MamI 2184 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 7407 CAATTG C^AATTG
MroI 3937 TCCGGA T^CCGGA
MunI 7407 CAATTG C^AATTG
NheI 7066 GCTAGC G^CTAGC
NsiI 4375 ATGCAT ATGCA^T
PflFI 2304 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 5317 TCATGA T^CATGA
RsaI 5878 GTAC GT^AC
Tth111I 2304 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 2498 GTGCAC G^TGCAC
6529 GTGCAC
ApaLI 2498 GTGCAC G^TGCAC
6529 GTGCAC
BbeI 1294 GGCGCC GGCGC^C
7233 GGCGCC
BcgI 847 CGAGGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4625 GCATGACTATCG
BciVI 7449 GGATAC GTATCC(6/5)
6395 GTATCC
BsiEI 3965 CGGCCG CG[GA][CT]^CG
5422 CGGCCG
BsmBI 5837 CGTCTC CGTCTC(1/5)
5915 GAGACG
BsrDI 1514 CATTGC GCAATG(2/0)
4609 GCAATG
EagI 3965 CGGCCG C^GGCCG
5422 CGGCCG
EclXI 3965 CGGCCG C^GGCCG
5422 CGGCCG
Eco52I 3965 CGGCCG C^GGCCG
5422 CGGCCG
KasI 1294 GGCGCC G^GCGCC
7233 GGCGCC
KspI 2505 CCGCGG CCGC^GG
2603 CCGCGG
NaeI 2168 GCCGGC GCC^GGC
2736 GCCGGC
NarI 1294 GGCGCC GG^CGCC
7233 GGCGCC
NdeI 796 CATATG CA^TATG
4576 CATATG
NgoMIV 2168 GCCGGC G^CCGGC
2736 GCCGGC
SacII 2505 CCGCGG CCGC^GG
2603 CCGCGG
SapI 3204 GAAGAGC GCTCTTC(1/4)
1403 GCTCTTC
SbfI 4087 CCTGCAGG CCTGCA^GG
5488 CCTGCAGG
SfiI 2721 GGCCCCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4686 GGCCGATGGGGCC
SfoI 1294 GGCGCC GGC^GCC
7233 GGCGCC
SphI 4373 GCATGC GCATG^C
6984 GCATGC
Sse8387I 4087 CCTGCAGG CCTGCA^GG
5488 CCTGCAGG
TaqI 2473 TCGA T^CGA
4782 TCGA
TthHB8I 2473 TCGA T^CGA
4782 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AflIII AhdI AluI AlwI AlwNI
AocI ApaI ApoI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eam1105I EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PshAI PstI PvuII SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII XmaCI XmaI