##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AccII AccIII AclI AgeI AhdI
AocI ApaI AscI AspEI AviII
AvrII BamHI BbeI BbrPI BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BsrBI
BsrFI BssHII Bst1107I BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT14I FseI FspI HgaI HincII
HindII HindIII HpaI KasI KspI
MamI MluI MroI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SnaBI Sse8387I StuI StyI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 528 GTCTAC GT^[AC][GT]AC
AflII 2207 CTTAAG C^TTAAG
AflIII 1792 ACATGT A^C[GA][CT]GT
Alw44I 237 GTGCAC G^TGCAC
Aor51HI 186 AGCGCT AGC^GCT
ApaLI 237 GTGCAC G^TGCAC
Asp700I 3911 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2908 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 223 TGGCCA TGG^CCA
BbvCI 3003 CCTCAGC CCTCAGC(-5/-2)
BciVI 1202 GTATCC GTATCC(6/5)
BclI 343 TGATCA T^GATCA
BfrI 2207 CTTAAG C^TTAAG
BglI 148 GCCACCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2950 AGATCT A^GATCT
BlpI 51 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 51 GCTGAGC GC^T[ACGT]AGC
BsaI 3105 GGTCTC GGTCTC(1/5)
BsmI 1537 GAATGC GAATGC(1/-1)
BspLU11I 1792 ACATGT A^CATGT
BspMI 1058 ACCTGC ACCTGC(4/8)
BssSI 3122 CTCGTG CACGAG(-5/-1)
BstAPI 1340 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 93 TTCGAA TT^CGAA
CelII 51 GCTGAGC GC^T[ACGT]AGC
CfoI 187 GCGC GCG^C
DrdI 136 GACGGCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 860 CTCTTC CTCTTC(1/4)
Eco47III 186 AGCGCT AGC^GCT
EcoT22I 1627 ATGCAT ATGCA^T
FbaI 343 TGATCA T^GATCA
HaeII 186 AGCGCT [GA]GCGC^[CT]
HhaI 187 GCGC GCG^C
HinP1I 187 GCGC G^CGC
Ksp632I 860 CTCTTC CTCTTC(1/4)
MluNI 223 TGGCCA TGG^CCA
MscI 223 TGGCCA TGG^CCA
NsiI 1627 ATGCAT ATGCA^T
NspV 93 TTCGAA TT^CGAA
PacI 3737 TTAATTAA TTAAT^TAA
PflFI 2908 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 279 AGGACCT [GA]G^G[AT]CC[CT]
ScaI 4076 AGTACT AGT^ACT
SfuI 93 TTCGAA TT^CGAA
SpeI 4013 ACTAGT A^CTAGT
Tth111I 2908 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2350 TCTAGA T^CTAGA
XcmI 2310 CCAAAGCAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3911 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 142 GACGTC GACGT^C
1481 GACGTC
Acc65I 176 GGTACC G^GTACC
3486 GGTACC
AcyI 142 GACGTC G[GA]^CG[CT]C
1481 GACGTC
AlwNI 327 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1067 CAGGCACTG
AseI 458 ATTAAT AT^TAAT
4144 ATTAAT
AsnI 458 ATTAAT AT^TAAT
4144 ATTAAT
Asp718I 176 GGTACC G^GTACC
3486 GGTACC
AspHI 237 GTGCAC G[AT]GC[AT]^C
696 GAGCAC
BbsI 1617 GAAGAC GAAGAC(2/6)
1152 GTCTTC
BcgI 2992 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3968 CGAATGTTTTGC
BpuAI 1617 GAAGAC GAAGAC(2/6)
1152 GTCTTC
BsaHI 142 GACGTC G[GA]^CG[CT]C
1481 GACGTC
BseRI 314 GAGGAG GAGGAG(10/8)
689 GAGGAG
BsiHKAI 237 GTGCAC G[AT]GC[AT]^C
696 GAGCAC
BsmBI 2906 GAGACG CGTCTC(1/5)
3075 GAGACG
BstXI 3147 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3333 CCAGTGATTTGG
DraII 279 AGGACCT [GA]G^G[ACGT]CC[CT]
3184 AGGCCCT
EaeI 223 TGGCCA [CT]^GGCC[GA]
1253 CGGCCA
EcoNI 1444 CCTAAGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1806 CCTTGCCCAGG
EcoO109I 279 AGGACCT [GA]G^G[ACGT]CC[CT]
3184 AGGCCCT
EcoRI 1876 GAATTC G^AATTC
3467 GAATTC
Hin1I 142 GACGTC G[GA]^CG[CT]C
1481 GACGTC
KpnI 176 GGTACC GGTAC^C
3486 GGTACC
MaeII 143 ACGT A^CGT
1482 ACGT
NdeI 1157 CATATG CA^TATG
3574 CATATG
PshBI 458 ATTAAT AT^TAAT
4144 ATTAAT
PstI 1910 CTGCAG CTGCA^G
2360 CTGCAG
PvuII 384 CAGCTG CAG^CTG
1643 CAGCTG
SmaI 25 CCCGGG CCC^GGG
172 CCCGGG
SphI 1411 GCATGC GCATG^C
3035 GCATGC
SspI 2568 AATATT AAT^ATT
4050 AATATT
XmaCI 25 CCCGGG C^CCGGG
172 CCCGGG
XmaI 25 CCCGGG C^CCGGG
172 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII