##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I AspI AviII
AvrII BcgI BciVI BclI BfrI
BlnI BsiWI BsmBI BspHI BspLU11I
BssSI Bst1107I BstBI BstZ17I CpoI
DraI EcoRI EcoRV FbaI FspI
HindIII HpaI MluI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
SfuI SnaBI Sse8387I SspI SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3813 GGTACC G^GTACC
AccI 3945 GTCTAC GT^[AC][GT]AC
AccIII 2814 TCCGGA T^CCGGA
AhdI 84 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2046 CCTCAGG CC^T[ACGT]AGG
Asp718I 3813 GGTACC G^GTACC
AspEI 84 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 456 TGGCCA TGG^CCA
BamHI 2884 GGATCC G^GATCC
BbrPI 3757 CACGTG CAC^GTG
BbvCI 4022 CCTCAGC CCTCAGC(-5/-2)
BglII 2748 AGATCT A^GATCT
BsaBI 358 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2569 GGTCTC GGTCTC(1/5)
BsaWI 2814 TCCGGA [AT]^CCGG[AT]
BseAI 2814 TCCGGA T^CCGGA
BsmI 2782 GAATGC GAATGC(1/-1)
BspDI 2636 ATCGAT AT^CGAT
BspEI 2814 TCCGGA T^CCGGA
BstEII 3061 GGTGACC G^GT[ACGT]ACC
BstPI 3061 GGTGACC G^GT[ACGT]ACC
Bsu36I 2046 CCTCAGG CC^T[ACGT]AGG
ClaI 2636 ATCGAT AT^CGAT
Eam1105I 84 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2046 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3061 GGTGACC G^GT[ACGT]ACC
EcoT22I 2897 ATGCAT ATGCA^T
FseI 154 GGCCGGCC GGCCGG^CC
HincII 3614 GTCAAC GT[CT]^[GA]AC
HindII 3614 GTCAAC GT[CT]^[GA]AC
KpnI 3813 GGTACC GGTAC^C
MamI 358 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3421 CAATTG C^AATTG
MluNI 456 TGGCCA TGG^CCA
MroI 2814 TCCGGA T^CCGGA
MscI 456 TGGCCA TGG^CCA
MunI 3421 CAATTG C^AATTG
NheI 1318 GCTAGC G^CTAGC
NsiI 2897 ATGCAT ATGCA^T
PmaCI 3757 CACGTG CAC^GTG
PmlI 3757 CACGTG CAC^GTG
ScaI 1197 AGTACT AGT^ACT
SexAI 2382 ACCAGGT A^CC[AT]GGT
SgrAI 1333 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 4109 CTCAAG C^T[CT][GA]AG
SpeI 3898 ACTAGT A^CTAGT
SphI 565 GCATGC GCATG^C
XbaI 3597 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BlpI 2866 GCTCAGC GC^T[ACGT]AGC
3985 GCTAAGC
Bpu1102I 2866 GCTCAGC GC^T[ACGT]AGC
3985 GCTAAGC
BsaAI 1355 TACGTG [CT]AC^GT[GA]
3757 CACGTG
Bsp1407I 549 TGTACA T^GTACA
3336 TGTACA
BsrBI 185 CCGCTC CCGCTC(-3/-3)
1903 GAGCGG
BsrDI 989 CATTGC GCAATG(2/0)
1611 GCAATG
BsrGI 549 TGTACA T^GTACA
3336 TGTACA
CelII 2866 GCTCAGC GC^T[ACGT]AGC
3985 GCTAAGC
NcoI 129 CCATGG C^CATGG
3350 CCATGG
NspI 565 GCATGC [GA]CATG^[CT]
2899 GCATGT
PflMI 124 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2875 CCACTGGGTGG
SapI 3405 GAAGAGC GCTCTTC(1/4)
3561 GAAGAGC
SfiI 145 GGCCGGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1876 GGCCTCCCGGGCC
SspBI 549 TGTACA T^GTACA
3336 TGTACA
Van91I 124 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2875 CCACTGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI