The cDNA fragment was inserted at the NotI-EcoRV site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The cDNA was cloned into the vector in the direction of SacI site to KpnI site of the vector. The NotI sites at the both 5'- and 3'-end of the cDNA were disrupted by the vector ligation. |
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