The cDNA fragment was inserted at the NotI site of the
pBluescript II SK(+) vector (GenBank Accession No.
X52328, AmpR). The 5' end of the cDNA (NotI site) was ligated 
with the NotI site of the vector without using any linker 
or adapter. The 3' end of the cDNA was inserted at the 
NotI site of the vector. The cDNA was cloned into the 
vector in the direction of SacI site to KpnI site of the 
vector.