The cDNA fragment was originally inserted at SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328) with the SalI adapter (5'TCGACCCACGCGTCCG3') and its XhoI-NotI DNA fragment was inserted at the XhoI-NotI site of the pYO326 vector (Amp-r). pYO326 shuttle vector was kindly provided by Y. Ohya (University of Tokyo). |
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