The cDNA fragment was inserted at the SalI-NotI site 
of the pYO326GW (Amp-r) vector. The 5' end of the cDNA was repaired and 
ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and 
inserted at the SalI site of the vector. The 3'end of the cDNA was 
inserted at the NotI site of the vector. The original pYO326 shuttle
vector (Amp-r) was kindly provided by Y. Ohya (University of Tokyo).