The cDNA fragment was originally inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR) without using any linker or adapter and a RT-PCR product (1-1405) was subcloned upstream of the cDNA (1406-2677). The adjacent sequence to the 5' end of the cDNA is as follows: GGGCCCCCCCTCGAGGTCGAGGCCGCGGGAATTCGATT-5' end of cDNA. |
Back |