The cDNA fragment was originally inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR) without using any linker or adapter and a RT-PCR product (1-180) was subcloned upstream of the cDNA (181-2657). The adjacent sequence to the 5' end of the cDNA is as follows: GGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTT-5' end of cDNA. |
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