The cDNA fragment was originally inserted at SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR) and a synthetic oligonucleotide (1-52) was subcloned upstream of the cDNA (53- 4602). The adjacent sequence to the 5' end of the cDNA is as follows: GGGCCCCCCCTCGAGGTCGACCCACGCGT-5' end of cDNA. |
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