The cDNA fragment was originally inserted at SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR) and a synthetic oligonucleotide (1-60) was subcloned upstream of the cDNA (61-4434). The adjacent sequence to the 5' end of the cDNA is as follows: GGGCCCCCCCTCGAGGTCGACCCAATACGCGT-5' end of cDNA. |
Back |