The cDNA fragment was originally inserted at SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR) and a RT-PCR product (1-2647) was subcloned upstream of the cDNA (2648-6719). The adjacent sequence to the 5' end of the cDNA is as follows: GGGCCCCCCCTCGAGGTCGACCCACGCGGGCCGCGGGAATTCGATT-5' end of cDNA. |
Back |