The cDNA fragment was originally inserted at SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR) and a RT-PCR product (1-289) was subcloned upstream of the cDNA (290-4444). The adjacent sequence to the 5' end of the cDNA is as follows: GGGCCCCCCCTCGAGGTCGACCCACGCGT-5' end of cDNA. |
Back |