The cDNA fragment was inserted at the SalI-SacI site of of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The adjacent sequence to the 5'/3' end of the cDNA is as follows: 5'-end: GGGCCCCCCCTCGAGGTCGACCCACGCGTCCG-5' end of cDNA 3'-end: GAGCTCTCACCACTTTGTACAAGAAAGCTGGGCGGCCGTTTTTTTTTTTTTTTTTT-3' end of cDNA |
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