!!NA_SEQUENCE 1.0
LOCUS AB032066 3297 bp DNA circular SYN 11-SEP-1999
DEFINITION Cloning vector pNTT104 DNA, complete sequence.
ACCESSION AB032066
VERSION AB032066.1 GI:5869538
KEYWORDS .
SOURCE Cloning vector pNTT104 (lab_host:Escherichia coli) DNA.
ORGANISM Cloning vector pNTT104
artificial sequence; vectors.
REFERENCE 1 (bases 1 to 3297)
AUTHORS Nomura,N. and Nagase,T.
TITLE The nucleotide sequence of the pNTT104 vector
JOURNAL Published Only in DataBase (1999) In press
REFERENCE 2 (bases 1 to 3297)
AUTHORS Nomura,N. and Nagase,T.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-1999) to the DDBJ/EMBL/GenBank databases. Nobuo
Nomura, Kazusa DNA Research Institute, Laboratory of Gene Structure
1; 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan
(E-mail:nomura@kazusa.or.jp, URL:http://www.kazusa.or.jp,
Tel:81-438-52-3932, Fax:81-438-52-3931)
COMMENT pNTT104
This sequence is derived from the pUC118 sequence mainly compiled
by changing the polylinker sequence (sequence at the position
between 231 and 291 of the pUC118) to the 194-bp sequence (sequence
at the position between 231 and 426 of the pNTT104) containing
multiple restriction enzyme recognition sites. In addition to that,
both the ScaI and NarI sites of the pUC118 were eliminated by
nucleotide exchanges at the positions 586 (C to T) and 1802 (G to
A) of the pNTT104 sequence, respectively.
FEATURES Location/Qualifiers
source 1. .3297
/organism="Cloning vector pNTT104"
/db_xref="taxon:103691"
/lab_host="Escherichia coli"
misc_feature 205. .462
/note="complementary base sequence was reported as
Acc#:D16587; polylinker"
BASE COUNT 789 a 851 c 818 g 839 t
ORIGIN
AB032066 Length: 3297 June 19, 2001 19:42 Type: N Check: 4932 ..
1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA
51 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA
101 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT
151 TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT
201 CACACAGGAA ACAGCTATGA CCATGATTAC GGCATGCCTG CAGGCGCCGA
251 CGTCATGCAT GGGCCCTCTA GATCTAGTAC TAGTGATATC GATCGGTCCG
301 TACGTTTTTC AGCACGCGTT AAAAAAACCA TCCCCCTGGA AGCTTAAGGC
351 CTCGAGTTCG AATTCGCGGC CGCACGTGTC CATGGATCCC GGGTCGACGG
401 CCGCGGTGGC CGGTACCGAG CTCATACTGG CCGTCGTTTT ACAACGTCGT
451 GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC
501 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT
551 CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTGAT GCGGTATTTT
601 CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGTC AAAGCAACCA
651 TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC
701 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG
751 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT
801 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT
851 CGACCCCAAA AAACTTGATT TGGGTGATGG TTCACGTAGT GGGCCATCGC
901 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT
951 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGGCTA
1001 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA
1051 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG
1101 TTTACAATTT TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA
1151 GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG
1201 CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG
1251 AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG
1301 AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA
1351 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC
1401 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG
1451 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA
1501 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC
1551 CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA
1601 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG
1651 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC
1701 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG
1751 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG
1801 AATACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA
1851 GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT
1901 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA
1951 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT
2001 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC
2051 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC
2101 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT
2151 CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC
2201 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA
2251 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG
2301 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA
2351 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA
2401 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT
2451 CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA
2501 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG
2551 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT
2601 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG
2651 CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC
2701 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC
2751 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT
2801 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG
2851 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG
2901 AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG
2951 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA
3001 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG
3051 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
3101 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
3151 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT
3201 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG
3251 CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAG