!!NA_SEQUENCE 1.0
LOCUS PSPORT1 4109 bp DNA SYN 06-DEC-1999
DEFINITION Cloning vector pSport1, complete cds.
ACCESSION U12390
VERSION U12390.1 GI:531828
KEYWORDS .
SOURCE Cloning vector pSport1.
ORGANISM Cloning vector pSport1
artificial sequence; vectors.
REFERENCE 1 (sites)
AUTHORS D'Alessio,J.M., Gruber,C.E., Cain,C. and Noon,M.C.
TITLE Construction of Directional cDNA Libraries Using the Superscript
Plasmid System
JOURNAL Focus 12, 47-50 (1990)
REFERENCE 2 (bases 1 to 4109)
AUTHORS Horton,M.E.
TITLE Direct Submission
JOURNAL Submitted (18-JUL-1994) Horton M. E., Life Technologies, Inc.,
Technical Services, 8400 Helgerman Court, Gaithersburg, MD
20884-9980, USA
FEATURES Location/Qualifiers
source 1. .4109
/organism="Cloning vector pSport1"
/db_xref="taxon:36586"
CDS complement(<1. .341)
/codon_start=1
/transl_table=11
/evidence=experimental
/product="beta-galactosidase alpha peptide"
/protein_id="AAA73456.2"
/db_xref="GI:6525246"
/translation="MTMITPSSNTTHYRESWYACRYRSGIPGSTSSLVGGRSRGSKLT
YACMRRHSSSIVSPKFNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR
PSQQLRSLNGE"
primer_bind complement(125. .147)
/standard_name="M13/pUC 23 base forward sequencing primer"
primer_bind complement(150. .161)
/standard_name="M13/pUC forward sequencing primer"
/evidence=experimental
promoter 164. .180
/note="SP6 promoter"
/evidence=experimental
misc_feature 191. .285
/function="multiple cloning site"
/evidence=experimental
promoter complement(298. .318)
/note="T7 promoter"
/evidence=experimental
CDS complement(464. .1546)
/codon_start=1
/transl_except=(pos:complement(1544. .1546),aa:Met)
/transl_table=11
/evidence=experimental
/product="lac repressor"
/protein_id="AAA73457.1"
/db_xref="GI:531830"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
rep_origin complement(1896. .1902)
/standard_name="ORI E. coli pMB1 (ColE1 and pBR322)"
/evidence=experimental
CDS complement(2661. .3521)
/function="ampicillin resistance"
/codon_start=1
/transl_table=11
/evidence=experimental
/product="beta-lactamase"
/protein_id="AAA73458.1"
/db_xref="GI:531831"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 3667. .4109
/note="F1 intergenic region"
/evidence=experimental
BASE COUNT 1002 a 1064 c 1062 g 981 t
ORIGIN
U12390 Length: 4109 May 26, 2000 16:28 Type: N Check: 1267 ..
1 CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
51 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT
101 TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
151 GCCAGTGAAT TGAATTTAGG TGACACTATA GAAGAGCTAT GACGTCGCAT
201 GCACGCGTAC GTAAGCTTGG ATCCTCTAGA GCGGCCGCCG ACTAGTGAGC
251 TCGTCGACCC GGGAATTCCG GACCGGTACC TGCAGGCGTA CCAGCTTTCC
301 CTATAGTGAG TCGTATTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC
351 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA
401 AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
451 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC
501 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT
551 GGGCGCCAGG GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT
601 TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG
651 GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG ATGGTGGTTG ACGGCGGGAT
701 ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC GAGATATCCG
751 CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC
801 ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG
851 CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC
901 GTTCCGCTAT CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA
951 GCCAGACGCA GACGCGCCGA GACAGAACTT AATGGGCCCG CTAACAGCGC
1001 GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC AGTCGCGTAC
1051 CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA
1101 TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC
1151 ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACCCGTTGCG
1201 CGAGAAGATT GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT
1251 ACCATCGACA CCACCACGCT GGCACCCAGT TGATCGGCGC GAGATTTAAT
1301 CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG GAGGTGGCAA
1351 CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC CACGCGGTTG
1401 GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT CCCGCGTTTT
1451 CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG
1501 AGACACCGGC ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC
1551 ACCACCCTGA ATTGACTCTC TTCCGGGCGC TATCATGCCA TACCGCGAAA
1601 GGTTTTGCGC CATTCGATGG TGTCAACGTA AATGCCGCTT CGCCTTCGCG
1651 CGCGAATTGC AAGCTCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
1701 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC
1751 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA
1801 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
1851 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT
1901 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
1951 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
2001 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
2051 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC
2101 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
2151 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA
2201 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
2251 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
2301 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT
2351 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
2401 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
2451 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT
2501 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
2551 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
2601 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT
2651 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
2701 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
2751 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC
2801 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
2851 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
2901 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA
2951 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
3001 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
3051 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC
3101 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
3151 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
3201 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC
3251 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
3301 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
3351 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC
3401 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
3451 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
3501 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA
3551 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
3601 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
3651 TGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT
3701 AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT
3751 AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA
3801 CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA
3851 CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT
3901 TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
3951 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG
4001 AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
4051 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
4101 GGGCGCGTC