The TAC Home Page

The TAC vector system for Agrobacterium-mediated plant transformation
with large DNA fragments

Updated at June 27, 2000, at March 26, 2008 by Daisuke Shibata of Kazusa DNA Res. Inst.

The transformation-competent bacterial artificial chromosome vector (TAC) was developed for plant transformation with large DNA fragments (Liu et al., 1999). An Arabidopsis genome DNA library that was constructed using the TAC vector had been contributing the Arabidopsis genome sequencing project of the chromosome III and V at Kazusa DNA Research Institute (Sato et al., 1998) and positional cloning of Arabidopsis genes (Liu et al, 1999; Sawa et al., 1999; unpublished data).

This homepage provides the information of the TAC vector system.
  1. General information of the TAC vector
  2. Plant Transformation of Arabidopsis with TAC clones
  3. Construction of the Arabidopsis genomic DNA library
  4. Protocols
  1. Shibata, D. & Liu, Y-G. (2000) "Agrobacterium-mediated plant transformation with large DNA fragments" Trends in Plant Science 5, 354-357
  2. Liu, Y.-G. et al. (1999) "Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning." Proc. Natl. Acad. Sci. U. S. A. 96, 6535-6540
  3. Sawa, S. et al. (1999) "FILAMENTOUS FLOWER, a meristem and organ identity gene of Arabidopsis, encodes a protein with a zinc finger and HMG-related domains. "Genes Dev. 13, 1079-1088 [see erratum: 13, 2337]
  4. Sato, S. et al. (1998) "A physical map of Arabidopsis thaliana chromosome 3 represented by two contigs of CIC YAC, P1, TAC and BAC clones" DNA Res. 5, 163-168

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