KIAA0027

Product ID	ORK00373
Accession No	D25217
Description	megalencephalic leukoencephalopathy with subcortical cysts 1, transcript variant 1
Clone name	fk03155
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3629 bp) Predicted protein sequence (418 aa)
HaloTag ORF Clone	FHC00373
Flexi ORF Clone	FXC00373
Source	Human fetal brain
Rouge ID	mKIAA0027 by Kazusa Mouse cDNA Project
Note	We replaced ha00522, former representative clones for KIAA0027 with fk03155. (2002/5/10)

Length: 3629 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Integrity of 3' end

Length of 3'UTR	2185 bp
Genome contig ID	gi89161203r_48739954
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- TGTTTAATACGCTTGAGCAATAAACGCTGACTTGC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGACGTGGCTGTGGCTATTTAATATTGTGTCTCAGCAGCGTCAGCTCACT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	22	r	48839954	48866161	12	99.4	Perfect prediction

Length: 418 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
AAH70042	1.5e-155	99.7	Megalencephalic...	Homo sapiens
AAH28425	1.5e-155	99.7	Megalencephalic...	Homo sapiens
Q60HE7	7.5e-152	96.8	Membrane protei...	Macaca fascicularis
EDL04387	1e-139	85.7	megalencephalic...	Mus musculus
EDL76527	5.7e-138	85.0	megalencephalic...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
ScanRegExp	IPR001209	125	147	PS00527	Ribosomal protein S14

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	94	VFSVLMGSCLLVTSGFSLYLGNV	116	PRIMARY	23
	2	121	MDYLRCAAGSCIPSAIVSFTVS	142	SECONDARY	22
	3	151	NFQILFVSTFAVTTTCLIWFGCK	173	PRIMARY	23
	4	184	NFNLILLLLLELLMAATVIIAAR	206	PRIMARY	23
	5	240	SVVEVIAGISAVLGGIIALNVDD	262	PRIMARY	23
	6	268	HLSVTFFWILVACFPSAIASHVA	290	PRIMARY	23
	7	302	LIAISSLTSPLLFTASGYLSFSI	324	PRIMARY	23
	8	338	IKPSYDVLLLLLLLVLLLQAGLN	360	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	TCAGGGGCCGGGCAAACACT
Primer_r	CTCAAAGCTGCAGGGAGGTG
PCR product length	363 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles