KIAA0199

Product ID	ORK00453
Accession No	D83782
Description	SREBF chaperone
Clone name	ha03150s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No.X52328, AmpR). The adjacent sequence to the 5' end of the cDNA is as follows: GGGCCCCCCC TCGAGGTCGA CCCACGCGTC CGCCCACGCG TCCG-5' end of cDNA.
cDNA sequence	DNA sequence (4226 bp) Predicted protein sequence (1283 aa)
HaloTag ORF Clone	FHC00453
Flexi ORF Clone	FXC00453
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0199 by Kazusa Mouse cDNA Project
Note	We replaced ha03150, former representative clones for KIAA0199 with ha03150s1. (2002/5/10)

Length: 4226 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	141 bp
Genome contig ID	gi89161205r_47330207
PolyA signal sequence (ATTAAA,-21)	+--------+--------+----*----+---- CTGACTGTAATAATATTAAACTTTTTTAAAAAACC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATATCATCATCTGTCAGGCACTTTGGGAGCTACTTGTGTCTCTTGGAATT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	3	r	47430207	47492439	23	100.0	Perfect prediction

Length: 1283 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q12770	100.0	Sterol regulato...	Homo sapiens
EAW64826	99.9	SREBP cleavage-...	Homo sapiens
XP_001499891	93.6	similar to Ster...	Equus caballus
A2RRU4	92.5	Sterol regulato...	Rattus norvegicus
A6QM06	92.5	Sterol regulato...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001680	1096	1110	PR00320	WD40 repeat
	IPR001680	1137	1151	PR00320	WD40 repeat
	IPR001680	1177	1191	PR00320	WD40 repeat
HMMPfam	IPR001680	770	806	PF00400	WD40 repeat
	IPR001680	1073	1109	PF00400	WD40 repeat
	IPR001680	1113	1150	PF00400	WD40 repeat
	IPR001680	1154	1190	PF00400	WD40 repeat
HMMSmart	IPR001680	769	806	SM00320	WD40 repeat
	IPR001680	959	997	SM00320	WD40 repeat
	IPR001680	1071	1109	SM00320	WD40 repeat
	IPR001680	1112	1150	SM00320	WD40 repeat
	IPR001680	1153	1190	SM00320	WD40 repeat
	IPR001680	1193	1230	SM00320	WD40 repeat
ProfileScan	IPR000731	288	446	PS50156	Sterol-sensing 5TM box
	IPR001680	1079	1239	PS50294	WD40 repeat
	IPR001680	1119	1159	PS50082	WD40 repeat
ScanRegExp	IPR001680	1137	1151	PS00678	WD40 repeat

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	25	LCASYPIPIILFTGFCILACCYP	47	PRIMARY	23
	2	284	IGVAELIPLVTTYIILFAYIYFS	306	PRIMARY	23
	3	319	LALAAVVTVLSSLLMSVGLCTLF	341	PRIMARY	23
	4	353	FPYLVVVIGLENVLVLTKSVVST	375	SECONDARY	23
	5	401	ATELGIILIGYFTLVPAIQEFCL	423	PRIMARY	23
	6	427	VGLVSDFFLQMLFFTTVLSIDI	448	SECONDARY	22
	7	714	KVAALGLATGIVLVLLLLCLYRV	736	PRIMARY	23
	8	776	LRGHLMDIECLASDGMLLVSCCL	798	SECONDARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	GCCTGGTGTATGTGCCCTCTG
Primer_r	CGGCTGGAAGATACTCGGCTC
PCR product length	140 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles