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Order Kazusa clone(s) from : |
| Product ID | ORK05608 |
|---|---|
| Accession No | AB011105 |
| Description | laminin, alpha 5 |
| Clone name | hg03784 |
| Vector information | |
| cDNA sequence | DNA sequence (5117 bp) Predicted protein sequence (1645 aa) |
| Source | Human adult brain |
| Rouge ID |
mKIAA0533
by Kazusa Mouse cDNA Project
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Length: 5117 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Length: 1645 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR002049 | 31 | 49 | PR00011 | EGF-like |
| IPR002049 | 74 | 92 | PR00011 | EGF-like | |
| HMMPfam | IPR002049 | 20 | 64 | PF00053 | EGF-like |
| IPR009254 | 141 | 401 | PF06008 | Laminin I | |
| IPR010307 | 582 | 712 | PF06009 | Laminin II | |
| IPR012680 | 920 | 1049 | PF02210 | Laminin G | |
| IPR012680 | 1103 | 1220 | PF02210 | Laminin G | |
| IPR012680 | 1320 | 1449 | PF02210 | Laminin G | |
| IPR012680 | 1499 | 1623 | PF02210 | Laminin G | |
| HMMSmart | IPR002049 | 20 | 64 | SM00180 | EGF-like |
| IPR002049 | 67 | 114 | SM00180 | EGF-like | |
| IPR001791 | 708 | 858 | SM00282 | Laminin G | |
| IPR001791 | 912 | 1049 | SM00282 | Laminin G | |
| IPR001791 | 1095 | 1220 | SM00282 | Laminin G | |
| IPR001791 | 1312 | 1449 | SM00282 | Laminin G | |
| IPR001791 | 1491 | 1623 | SM00282 | Laminin G | |
| ProfileScan | IPR002049 | 1 | 19 | PS50027 | EGF-like |
| IPR002049 | 20 | 66 | PS50027 | EGF-like | |
| IPR002049 | 67 | 116 | PS50027 | EGF-like | |
| IPR001791 | 686 | 879 | PS50025 | Laminin G | |
| IPR001791 | 891 | 1065 | PS50025 | Laminin G | |
| IPR001791 | 1074 | 1242 | PS50025 | Laminin G | |
| IPR001791 | 1290 | 1463 | PS50025 | Laminin G | |
| IPR001791 | 1470 | 1642 | PS50025 | Laminin G | |
| ScanRegExp | IPR001368 | 1 | 40 | PS00652 | TNFR/CD27/30/40/95 cysteine-rich region |
| IPR013032 | 20 | 31 | PS00022 | EGF-like region | |
| IPR013032 | 38 | 49 | PS00022 | EGF-like region | |
| IPR002049 | 38 | 69 | PS01248 | EGF-like | |
| IPR013032 | 81 | 92 | PS00022 | EGF-like region |
RT-PCR
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|---|
Experimental conditions| Primer_f | AGTTGCAGGTGACCGAGAAGC |
|---|---|
| Primer_r | CATTCCCGCTTTTCATCACCG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 20
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | AGTTGCAGGTGACCGAGAAGC |
| Primer_r | CATTCCCGCTTTTCATCACCG |
| PCR product length | 135 (0.4k) bp |
| PCR conditions | 95 °C 15 sec 66 °C 60 sec 30 cycles |