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Gene/Protein Characteristic Table for KIAA0597

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Product ID	ORK01100
Accession No	AB011169
Description	membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase, transcript variant 1
Clone name	hj02980
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4611 bp) Predicted protein sequence (971 aa)
HaloTag ORF Clone	FHC01100
Flexi ORF Clone	FXC01100
Source	Human adult brain
Rouge ID	mKIAA0597 by Kazusa Mouse cDNA Project

Length: 4611 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 971 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001841	70	116	PF00097	Zinc finger
HMMSmart	IPR011016	69	117	SM00744	Zinc finger

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	2	SLAFCQPLSLSLSPLLPLASSLA	24	SECONDARY	23
	2	154	WFHYTLVAFAWLGVVPLTACRIY	176	PRIMARY	23
	3	206	QGCFVVTCTLCAFISLVWLREQI	228	PRIMARY	23
	4	342	DGSLVFLEHVFWVVSLNTLFILV	364	PRIMARY	23
	5	394	LITTIVGYILLAITLIICHGLAT	416	PRIMARY	23
	6	435	KVSLLVVVEIGVFPLICGWWLDI	457	PRIMARY	23
	7	489	GMVYVFYFASFILLLREVLRPGV	511	PRIMARY	23
	8	541	FILSVIVFGSIVLLMLWLPIRII	563	PRIMARY	23
	9	583	VSELSLELLLLQVVLPALLEQGH	605	SECONDARY	23
	10	693	RIFLLIVFMCITLLIASLICLTL	715	PRIMARY	23
	11	738	TAACGLYVCWLTIRAVTVMVAWM	760	PRIMARY	23
	12	781	TLIVAVLLAGVVPLLLGLLFELV	803	PRIMARY	23
	13	822	QDWALGVLHAKIIAAITLMGPQW	844	SECONDARY	23
	14	875	ISVLLLSLCVPYVIASGVVPLLG	897	PRIMARY	23
	15	910	IYPFLLMVVVLMAILSFQVRQF	931	PRIMARY	22

RT-PCR

Experimental conditions

Chromosome No. 5
Experimental conditions

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