KIAA0598

Product ID	ORK01101
Accession No	AB011170
Description	carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15, transcript variant 1
Clone name	hj03045
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4712 bp) Predicted protein sequence (614 aa)
HaloTag ORF Clone	FHC01101
Flexi ORF Clone	FXC01101
Source	Human adult brain
Rouge ID	mKIAA0598 by Kazusa Mouse cDNA Project

Length: 4712 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2445 bp
Genome contig ID	gi89161187r_125657210
PolyA signal sequence (ATTAAA,-24)	+--------+--------+----*----+---- TATTTAACATAATTAAAGATGGACCCATAAGAGTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACGCCTGTGGAGCGCGTGCTCTTCCTCTGCAGCCAAGCTCTTCCTGGTGC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	10	r	125757210	125841898	8	100.0	Perfect prediction

Length: 614 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q7LFX5	100.0	N-acetylgalacto...	Homo sapiens
XP_521649	99.5	B cell RAG asso...	Pan troglodytes
XP_001082290	98.6	similar to B ce...	Macaca mulatta
EDM11711	92.7	N-acetylgalacto...	Rattus norvegicus
Q8CHI9	92.5	N-acetylgalacto...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000863	306	608	PF00685	Sulphotransferase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	5	CLFLYLCFPSIFCDHKAFLRFSS	27	SECONDARY	23
SOSUI2	2	133	CSLVFGLIIMTLVMASYILSGAH	155	PRIMARY	23

Primer_f	AAAGGGTTGTGTCCAGAGCGG
Primer_r	CGTGTGGGTGAGTGCGTCTTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	AAAGGGTTGTGTCCAGAGCGG
Primer_r	CGTGTGGGTGAGTGCGTCTTC
PCR product length	77 bp
PCR conditions	95 °C15 sec68 °C60 sec30 cycles