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Order Kazusa clone(s) from : |
| Product ID | ORK00634 |
|---|---|
| Accession No | AB018321 |
| Description | ATPase, Na+/K+ transporting, alpha 2 polypeptide |
| Clone name | fh12074 |
| Vector information | |
| cDNA sequence | DNA sequence (5411 bp) Predicted protein sequence (1049 aa) |
|
HaloTag ORF Clone |
FHC00634
|
| Flexi ORF Clone | FXC00634 |
| Source | Human fetal brain |
| Rouge ID |
mKIAA0778
by Kazusa Mouse cDNA Project
|
| Note | We replaced hk05292, former representative clones for KIAA0778 with fh12074. (2002/5/10) |
Length: 5411 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 2259 bp |
|---|---|
| Genome contig ID | gi89161185f_158252187 |
| PolyA signal sequence (AATAAA,-22) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (127811 - 127860) |
----+----*----+----*----+----*----+----*----+----* |
Ensembl ContigView (Add our DAS server as a DAS source)
| Chr | f/r | start | end | exon | identity | class | |
|---|---|---|---|---|---|---|---|
|
| 1 | f | 158352187 | 158379996 | 23 | 99.5 | Perfect prediction |
Length: 1049 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR006069 | 130 | 144 | PR00121 | ATPase |
| IPR006069 | 157 | 177 | PR00121 | ATPase | |
| IPR001757 | 241 | 255 | PR00119 | ATPase | |
| IPR006069 | 321 | 343 | PR00121 | ATPase | |
| IPR006069 | 394 | 415 | PR00121 | ATPase | |
| IPR001757 | 401 | 415 | PR00119 | ATPase | |
| IPR006069 | 531 | 549 | PR00121 | ATPase | |
| IPR001757 | 613 | 624 | PR00119 | ATPase | |
| IPR001757 | 635 | 645 | PR00119 | ATPase | |
| IPR001757 | 741 | 760 | PR00119 | ATPase | |
| IPR001757 | 765 | 777 | PR00119 | ATPase | |
| IPR006069 | 813 | 834 | PR00121 | ATPase | |
| IPR006069 | 880 | 900 | PR00121 | ATPase | |
| IPR006069 | 942 | 962 | PR00121 | ATPase | |
| IPR006069 | 976 | 1000 | PR00121 | ATPase | |
| HMMPfam | IPR004014 | 60 | 143 | PF00690 | ATPase |
| IPR008250 | 162 | 393 | PF00122 | E1-E2 ATPase-associated region | |
| IPR005834 | 397 | 764 | PF00702 | Haloacid dehalogenase-like hydrolase | |
| IPR006068 | 860 | 1038 | PF00689 | ATPase | |
| HMMTigr | IPR005775 | 54 | 1049 | TIGR01106 | ATPase |
| IPR001757 | 162 | 254 | TIGR01494 | ATPase | |
| IPR001757 | 306 | 431 | TIGR01494 | ATPase | |
| IPR001757 | 610 | 660 | TIGR01494 | ATPase | |
| IPR001757 | 715 | 834 | TIGR01494 | ATPase | |
| ScanRegExp | IPR001757 | 403 | 409 | PS00154 | ATPase |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 2 | RLSQTGWCGLGILLVTSPAKVPQ | 24 | SECONDARY | 23 | 2 | 125 | GGFSILLWIGAILCFLAYGIQAA | 147 | PRIMARY | 23 | 3 | 157 | LYLGVVLAAVVIVTGCFSYYQ | 177 | PRIMARY | 21 | 4 | 319 | IQLITGVAVFLGVSFFVLSLILG | 341 | PRIMARY | 23 | 5 | 351 | LIGIIVANVPEGLLATVTVCLTL | 373 | PRIMARY | 23 | 6 | 821 | IANIPLPLGTVTILCIDLGTDMV | 843 | SECONDARY | 23 | 7 | 941 | EFTCHTAFFASIVVVQWADLIIC | 963 | SECONDARY | 23 | 8 | 981 | IFGLLEETALAAFLSYCPGMGVA | 1003 | SECONDARY | 23 | 9 | 1009 | LKVTWWFCAFPYSLLIFIYDEV | 1030 | PRIMARY | 22 |
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RT-PCR-ELISA
|
Experimental conditions| Primer_f | TGGAGATTACTAACTGTGGAC |
|---|---|
| Primer_r | GATAATTAGCCCATGCACTCG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 1
Experimental conditions| Panel name | UniGene |
|---|---|
| Primer_f | - |
| Primer_r | - |
| PCR product length | - |
| PCR conditions | - |