KIAA1825

Product ID	ORK01182
Accession No	AB058728
Description	ATPase type 13A1
Clone name	fj04975
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3832 bp) Predicted protein sequence (1203 aa)
HaloTag ORF Clone	FHC01182
Flexi ORF Clone	FXC01182
Source	Human fetal brain
Rouge ID	mKIAA1825 by Kazusa Mouse cDNA Project

Length: 3832 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	219 bp
Genome contig ID	gi42406306r_19517012
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- GCACCTTTGGTAAATAAAGCAGCATCTGAGATTTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAAGCCCTGCCTCCACCTCGTCTGTGCCACACGGTGTTTGCTGGGGGC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	19	r	19617012	19635473	26	100.0	Perfect prediction

Length: 1203 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9HD20	100.0	Probable cation...	Homo sapiens
XP_533862	96.4	similar to Prob...	Canis lupus fam...
XP_001500954	95.8	ATPase type 13A...	Equus caballus
EDL28752	95.2	ATPase type 13A...	Mus musculus
Q9EPE9	95.1	Probable cation...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037768	1.2e-06	24.1	KIAA1347
AB018321	0.0003	23.0	KIAA0778
AB014603	0.0003	22.2	KIAA0703

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	347	361	PR00119	ATPase
	IPR001757	530	544	PR00119	ATPase
	IPR001757	738	748	PR00119	ATPase
	IPR001757	861	880	PR00119	ATPase
HMMPfam	IPR008250	301	391	PF00122	E1-E2 ATPase-associated region
	IPR005834	526	753	PF00702	Haloacid dehalogenase-like hydrolase
	IPR005834	835	884	PF00702	Haloacid dehalogenase-like hydrolase
HMMTigr	IPR006544	84	1173	TIGR01657	P-type ATPase of unknown pump specificity (type V)
	IPR001757	267	361	TIGR01494	ATPase
	IPR001757	444	547	TIGR01494	ATPase
	IPR001757	832	884	TIGR01494	ATPase
ScanRegExp	IPR001757	532	538	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	240	RATAPFFVFQVFCVGLWCLDEYW	262	PRIMARY	23
	2	264	YSVFTLSMLVAFEASLVQQQMRN	286	SECONDARY	23
	3	436	VTANNLETFIFILFLLVFAIAAA	458	PRIMARY	23
	4	492	LPIELSLAVNTSLIALAKLYMYC	514	SECONDARY	23
	5	983	TLQMFKILALNALILAYSQSVLY	1005	SECONDARY	23
	6	1011	FSDFQATLQGLLLAGCFLFISR	1032	SECONDARY	22
	7	1048	IFNLYTILTVMLQFFVHFLSLVY	1070	SECONDARY	23
	8	1095	PSLVNSTVYIMAMAMQMATFAIN	1117	SECONDARY	23
	9	1131	KPLVWSLAVSLLAIIGLLLGSS	1152	PRIMARY	22
	10	1166	VEFKLVIAQVLLLDFCLALLADR	1188	PRIMARY	23

Primer_f	ATTGTGAAACTGGGGGATGCC
Primer_r	ATCTTGAACATCTGTAGCGTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-