KIAA0810

Product ID	ORK07289
Accession No	AB018353
Description	Sad1 and UNC84 domain containing 1
Clone name	hk05647s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4047 bp) Predicted protein sequence (824 aa)
Source	Human adult brain
Rouge ID	mKIAA0810 by Kazusa Mouse cDNA Project
Note	We replaced hk05647, former representative clones for KIAA0810 with hk05647s1. (2002/12/27)

Length: 4047 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1571 bp
Genome contig ID	gi89161213f_738664
PolyA signal sequence (AATAAA,-27)	+--------+--------+----*----+---- GAGGTTTAAATAAAGAGTTTTAATTTTTAAAAGGG
Flanking genome sequence (142403 - 142452)	----+--------+--------+--------+--------+----* CATGGGATATAAACAGTCTATTTCTTTTCTCAGTTTGTCTGTTTCTGCCC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	7	f	838657	881065	21	99.7	Terminal No-hit

Length: 824 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAI52419	100.0	UNC84A protein ...	Homo sapiens
O94901	99.8	Protein unc-84 ...	Homo sapiens
XP_001144264	98.2	unc-84 homolog ...	Pan troglodytes
EAW87177	88.5	unc-84 homolog ...	Homo sapiens
EAL23707	88.6	unc-84 homolog ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB014568	3.4e-44	34.6	KIAA0668

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR012919	687	822	PF07738	Sad1/UNC-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	252	WHIWACAGYFLLQILRRIGAVGQ	274	SECONDARY	23
	2	282	SALWLAVVAPGKAASGVFWWLGI	304	PRIMARY	23
	3	317	NVFLLTRCLRNICKFLVLLIPLF	339	PRIMARY	23

Primer_f	GCAGCAGAAGCACTACCAAAG
Primer_r	ACTGATTTGGGATTTGGGGTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GCAGCAGAAGCACTACCAAAG
Primer_r	ACTGATTTGGGATTTGGGGTG
PCR product length	130 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles