KIAA0877

Product ID	ORK00140
Accession No	AB020684
Description	dpy-19-like 1 (C. elegans)
Clone name	hk07357
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4436 bp) Predicted protein sequence (580 aa)
HaloTag ORF Clone	FHC00140
Flexi ORF Clone	FXC00140
Source	Human adult brain

Length: 4436 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2692 bp
Genome contig ID	gi89161213r_34835039
PolyA signal sequence (ATTAAA,-23)	+--------+--------+----*----+---- TATATATGTTAAATTAAAAAAAATCATGAGAAATG
Flanking genome sequence (99980 - 99931)	----+--------+--------+--------+--------+----* AGGGAAGTGTTTTTCTTGATTGTTATTTTCATTTTCACCTTGATTGTGAT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	7	r	34935019	35019750	19	99.4	Perfect prediction

Length: 580 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW94039	100.0	hCG1645499, iso...	Homo sapiens
Q2PZI1	100.0	Protein dpy-19 ...	Homo sapiens
XP_001169037	99.8	dpy-19-like 1 i...	Pan troglodytes
XP_001169062	99.8	dpy-19-like 1 i...	Pan troglodytes
XP_001500017	97.4	similar to Prot...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	10	FNLYPEVILASWYRIYTKIMDLI	32	SECONDARY	23
	2	58	GDPACFYVAVIFILNGLMMALFF	80	PRIMARY	23
	3	119	SFSYPFLVLQMLLVTHILRATKL	141	SECONDARY	23
	4	155	FFMLPWQFAQFVLLTQIASLFAV	177	PRIMARY	23
	5	191	IIYIHMISLALCFVLMFGNSMLL	213	PRIMARY	23
	6	216	YYASSLVIIWGILAMKPHFLKIN	238	PRIMARY	23
	7	240	SELSLWVIQGCFWLFGTVILKYL	262	PRIMARY	23
	8	314	TLLLPVVLVVFVAIVRKIISDMW	336	PRIMARY	23
	9	363	LLAYTALGILIMRLKLFLTPHMC	385	PRIMARY	23
	10	400	LFCKVHPGAIVFAILAAMSIQGS	422	PRIMARY	23

Primer_f	TAGCAGGTTCCATAGGCGTAC
Primer_r	CTTTGCCCATCCTTACTTTCC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	UniGene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-