KIAA0886

Product ID	ORK00673
Accession No	AB020693
Description	reticulon 4, transcript variant 1
Clone name	hk07722
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4053 bp) Predicted protein sequence (1233 aa)
HaloTag ORF Clone	FHC00673
Flexi ORF Clone	FXC00673
Source	Human adult brain
Rouge ID	mKIAA0886 by Kazusa Mouse cDNA Project

Length: 4053 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	340 bp
Genome contig ID	gi89161199r_54953452
PolyA signal sequence (None)	+--------+--------+----*----+---- CGCATCTTGGCAAGTTGCAGAGATGGTGGAGCTAG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAGCCCTTTTCAGTTTGTGCACTGTGTATGGTCCGT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	2	r	55053452	55131074	9	99.8	Perfect prediction

Length: 1233 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9NQC3	100.0	Reticulon-4; Ne...	Homo sapiens
CAB99248	99.9	Nogo-A protein ...	Homo sapiens
XP_001112090	95.7	reticulon 4 [Ma...	Macaca mulatta
EAX00129	98.4	reticulon 4, is...	Homo sapiens
AAM64244	100.0	RNT4 [Homo sapi...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003388	1046	1231	PF02453	Reticulon
ProfileScan	IPR003388	1046	1233	PS50845	Reticulon

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1047	VDLLYWRDIKKTGVVFGASLFLL	1069	SECONDARY	23
	2	1075	FSIVSVTAYIALALLSVTISFRI	1097	PRIMARY	23
	3	1150	LFLVDDLVDSLKFAVLMWVFTYV	1172	PRIMARY	23
	4	1176	FNGLTLLILALISLFSVPVIYER	1198	PRIMARY	23

Primer_f	GATTATACGGGGGAGGGTCAG
Primer_r	ACACATGGCAGTCAAGACAGG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GATTATACGGGGGAGGGTCAG
Primer_r	ACACATGGCAGTCAAGACAGG
PCR product length	131 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles