KIAA1059

Product ID	ORK00727
Accession No	AB028982
Description	sortilin-related VPS10 domain containing receptor 3
Clone name	hh12158
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5757 bp) Predicted protein sequence (1297 aa)
HaloTag ORF Clone	FHC00727
Flexi ORF Clone	FXC00727
Source	Human adult brain

Length: 5757 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1861 bp
Genome contig ID	gi89161187f_106290849
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- TGAAATTGTTGAAAATAAATGTATTTTTGTACATC
Flanking genome sequence (724136 - 724185)	----+--------+--------+--------+--------+----* AAAGCTACATACGTGGCTGAGTTTTCTCTTTGGGGAGTTTTCTTTATCCA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	10	f	106390849	107014983	26	100.0	Internal No-hit

Length: 1297 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAI17418	99.9	Sortilin-relate...	Homo sapiens
XP_508024	95.9	VPS10 domain re...	Pan troglodytes
XP_609898	93.9	similar to VPS1...	Bos taurus
Q8VI51	92.4	VPS10 domain-co...	Mus musculus
XP_001916849	95.2	similar to Sort...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037750	1e-89	45.0	KIAA1329

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002860	306	317	PF02012	Glycoside hydrolase
	IPR002860	354	365	PF02012	Glycoside hydrolase
	IPR002860	395	406	PF02012	Glycoside hydrolase
	IPR002860	590	601	PF02012	Glycoside hydrolase
	IPR002860	667	678	PF02012	Glycoside hydrolase
	IPR002860	709	720	PF02012	Glycoside hydrolase
	IPR000601	901	985	PF00801	PKD
HMMSmart	IPR006581	294	896	SM00602	VPS10
ProfileScan	IPR000601	935	979	PS50093	PKD

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1172	LKPGVQVIVYVTQLTLAPLVDSS	1194	SECONDARY	23
SOSUI2	2	1200	SAMLMLLSVVFVGLAVFLIYK	1220	PRIMARY	21

Primer_f	GTTCCCATTCTTCTTTGTGAG
Primer_r	ATTGTCTGCCCCATTTAACCC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GTTCCCATTCTTCTTTGTGAG
Primer_r	ATTGTCTGCCCCATTTAACCC
PCR product length	132 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles