KIAA1605

Product ID	ORK00875
Accession No	AB046825
Description	glucosidase, beta (bile acid) 2
Clone name	fj10791
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3757 bp) Predicted protein sequence (922 aa)
HaloTag ORF Clone	FHC00875
Flexi ORF Clone	FXC00875
Source	Human fetal brain
Rouge ID	mKIAA1605 by Kazusa Mouse cDNA Project

Length: 3757 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	609 bp
Genome contig ID	gi89161216r_35626864
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- AGACACATGGGTACAAATAAAAGACCCAGAAAGCC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATTAAGTGTGGTGTGTTTGAGCCCTCAGTCGTCCTGACAGGGCACAGGAG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	9	r	35726864	35739215	17	100.0	Perfect prediction

Length: 922 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
CAI10983	100.0	glucosidase, be...	Homo sapiens
XP_001167830	99.5	bile acid beta-...	Pan troglodytes
Q9HCG7	99.5	Non-lysosomal g...	Homo sapiens
XP_001167862	97.2	bile acid beta-...	Pan troglodytes
XP_520565	99.0	bile acid beta-...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR006775	566	915	PF04685	Protein of unknown function DUF608
ScanRegExp	IPR002202	199	206	PS00318	Hydroxymethylglutaryl-coenzyme A reductase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	831	FELNVQAFAGGAMGAVNGMQPH	852	SECONDARY	22
SOSUI2	2	871	YGLAATMIQELLPSGFCLWVIVI	893	PRIMARY	23

Primer_f	TTACAACTATGACAGCAGCTC
Primer_r	GATAGTTTGGAGAGCACGGAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-