KIAA1613

Product ID	ORK00878
Accession No	AB046833
Description	solute carrier family 7, member 14
Clone name	fj12845
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4573 bp) Predicted protein sequence (686 aa)
Source	Human fetal brain
Rouge ID	mKIAA1613 by Kazusa Mouse cDNA Project

Length: 4573 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2511 bp
Genome contig ID	gi89161205r_171565047
PolyA signal sequence (GATAAA,-31)	+--------+--------+----*----+---- AGTTGATAAATCACCTCCCTAGCAACCTTAGTTTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAAAAAAATCACTAAGATAGAAAAAGTATTTTCAGGTACATAGACATG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	3	r	171665047	171727186	7	99.4	Perfect prediction

Length: 686 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAH22968	100.0	SLC7A14 protein...	Homo sapiens
EAW78510	100.0	solute carrier ...	Homo sapiens
XP_526378	99.9	solute carrier ...	Pan troglodytes
Q8TBB6	99.9	Probable cation...	Homo sapiens
XP_001088713	99.1	similar to solu...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
D87432	6.7e-06	21.5	KIAA0245

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004841	4	352	PF00324	Amino acid permease-associated region

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	6	GPGVIVSFIIAAVASILSGVCYA	28	PRIMARY	23
	2	51	EFVAFFIGWNLILEYLIGTAAGA	73	PRIMARY	23
	3	109	YPDLLALLIAVIVTIIVALGVKN	131	PRIMARY	23
	4	138	VLNVLNLAVWVFIMIAGLFFIN	159	PRIMARY	22
	5	177	LQGAATCFYAFIGFDIIATTGE	198	SECONDARY	22
	6	211	ITASLVICLTAYVSVSVILTLMV	233	PRIMARY	23
	7	260	VAIGSVAGLTVSLLGSLFPMPRV	282	SECONDARY	23
	8	305	TPVVACIVSGFLAALLALLVSLR	327	PRIMARY	23
	9	332	MMSIGTLLAYTLVSVCVLLLRY	353	PRIMARY	22
	10	485	HTVTICVLLLFILMFIFCSFIIF	507	PRIMARY	23
	11	517	WWAILLVVLMVLLISTLVFVILQ	539	PRIMARY	23
	12	548	PYMAPCLPFVPAFAMLVNIYLML	570	PRIMARY	23
	13	575	ITWIRFAVWCFVGLLIYFGYGIW	597	PRIMARY	23

Primer_f	GGCAAAATCTTAACGTGGGTG
Primer_r	ACTATGTGGGAAAAATGGCTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-