KIAA1624

Product ID	ORK00258
Accession No	AB046844
Description	G protein-coupled receptor 107, transcript variant 1
Clone name	fg02331
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6840 bp) Predicted protein sequence (599 aa)
HaloTag ORF Clone	FHC00258
Flexi ORF Clone	FXC00258
Source	Human fetal brain
Rouge ID	mKIAA1624 by Kazusa Mouse cDNA Project

Length: 6840 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Integrity of 3' end

Length of 3'UTR	5039 bp
Genome contig ID	gi89161216f_131756035
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- CGTGTTGCAAATGTGTAAAATAAAGATTATCTGGC
Flanking genome sequence (186227 - 186276)	----+--------+--------+--------+--------+----* AGAACCGTGTGTGTTTGAGATGCAGGGAGTTGCCGGCTCCTGGTGCTGTC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	9	f	131856035	131942260	20	99.9	Perfect prediction

Length: 599 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAG11346	0	100.0	G-protein coupl...	synthetic construct
Q5VW38	0	99.7	Protein GPR107;...	Homo sapiens
EAW87922	3.1e-195	95.2	G protein-coupl...	Homo sapiens
XP_001164765	3.6e-195	95.0	G protein-coupl...	Pan troglodytes
AAK57695	4.3e-186	92.0	lung seven tran...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR009637	214	552	PF06814	Transmembrane receptor

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	19	GLRLLPMLGLLQLLAEPGLGRVH	41	SECONDARY	23
	2	263	KLYISMAFFFFLSGTIWIHIL	283	PRIMARY	21
	3	329	AVVYYITHLLKGALLFITIALIG	351	PRIMARY	23
	4	368	IFMIVIPLQVLANVAYIIIESTE	390	SECONDARY	23
	5	401	DSLFLVDLLCCGAILFPVVWSIR	423	PRIMARY	23
	6	496	YYVLIVCYIYFTRIIAFLLKLAV	518	PRIMARY	23
	7	523	KWLYQLLDETATLVFFVLTGYK	544	SECONDARY	22

Primer_f	CATTTGCTTTCCTGGTTAGAG
Primer_r	CAACACGGCAAGAGAATTCAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-