KIAA1691

Product ID	ORK00888
Accession No	AB051478
Description	tweety family member 3
Clone name	fj00597s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4816 bp) Predicted protein sequence (558 aa)
HaloTag ORF Clone	FHC00888
Flexi ORF Clone	FXC00888
Source	Human fetal brain
Rouge ID	mKIAA1691 by Kazusa Mouse cDNA Project
Note	We replaced fj00597, former representative clones for KIAA1691 with fj00597s1. (2002/5/10)

Length: 4816 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	3062 bp
Genome contig ID	gi89161213f_2538194
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- TTGGGTTTCATGTTTTAAAATAAATTTTAAAAAGC
Flanking genome sequence (132768 - 132817)	----+--------+--------+--------+--------+----* AAGCCTCTCTTCCTGAGCTGCTTTGGCTCCTGGCTGGGCGCTGGTAGGGA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	7	f	2638134	2670960	14	99.3	Perfect prediction

Length: 558 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9C0H2	0	100.0	Protein tweety ...	Homo sapiens
EAW87268	2e-208	96.9	tweety homolog ...	Homo sapiens
EDL89726	4.4e-207	94.8	tweety homolog ...	Rattus norvegicus
Q6P5F7	1.9e-206	94.8	Protein tweety ...	Mus musculus
BAC34944	5.4e-206	94.6	unnamed protein...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR006990	60	465	PF04906	Tweety

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	32	PGPAMAGVSYAAPWWVSLLHRLP	54	SECONDARY	23
	2	78	LLLLGAAALACLALDLLFLLFYS	100	PRIMARY	23
	3	118	CCCTAWCVIIATLVCSAGIAVG	139	PRIMARY	22
	4	210	LETLLGYTAAIPFWRNTAVSLEV	232	SECONDARY	23
	5	244	RWLGYLGLLLLDVIICLLVLVGL	266	PRIMARY	23
	6	275	VGVCLLGVLALVISWGALGLELA	297	PRIMARY	23
	7	423	LIYLALFSFVTALMFSSIVCSVP	445	PRIMARY	23

Primer_f	CTGAGGCCTGAACCATTTTGC
Primer_r	CCCAAGCTGAATAGAAACCAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-