KIAA1742

Product ID	ORK04779
Accession No	AB051529
Description	dispatched homolog 2 (Drosophila)
Clone name	pj01304s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5035 bp) Predicted protein sequence (1301 aa)
Source	Human brain (hippocampus)
Rouge ID	mKIAA1742 by Kazusa Mouse cDNA Project
Note	We replaced pj01304, former representative clones for KIAA1742 with pj01304s1. (2002/5/10)

Length: 5035 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Integrity of 3' end

Length of 3'UTR	737 bp
Genome contig ID	gi51511731f_38337773
PolyA signal sequence (AATAAA,-31)	+--------+--------+----*----+---- TGTTAATAAACAGTAATAATCCTTTCCATCTCTGC
Flanking genome sequence (112777 - 112826)	----+--------+--------+--------+--------+----* ACATTTTAGTCTCCTTGGAATCTATCTCACTACTTCACTACAGGGTAGCC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	15	f	38437726	38450548	8	98.9	Perfect prediction

Length: 1301 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
A7MBM2	100.0	Protein dispatc...	Homo sapiens
EAW92408	100.0	dispatched homo...	Homo sapiens
XP_523050	99.5	dispatched B [P...	Pan troglodytes
XP_544618	89.8	similar to disp...	Canis lupus fam...
XP_001501244	89.6	dispatched homo...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
ProfileScan	IPR000731	407	543	PS50156	Sterol-sensing 5TM box

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	71	AVLMLCLAVIFLCTLAGLLGARL	93	PRIMARY	23
	2	391	YPLLALVAIFFGMALYLRSLFLT	413	PRIMARY	23
	3	418	LGVLGSLLVAFFLYQVAFRMAYF	440	PRIMARY	23
	4	445	LAALLLLSSVCANHTLIFFDLWR	467	PRIMARY	23
	5	485	TMHHFGYLLLVSGLTTSAAFYAS	507	SECONDARY	23
	6	520	LFMGTAVLVHLALTLVWLPASAV	542	PRIMARY	23
	7	860	LSTEPAVVLGLALALAFATLLLG	882	PRIMARY	23
	8	886	VPLSLFSVAAVAGTVLLTVGLLV	908	PRIMARY	23
	9	916	TAEALFLSASVGLSVDFTVNYCI	938	SECONDARY	23
	10	961	CATAVGAAALFAAGVLMLPATVL	983	PRIMARY	23
	11	992	LMMVKCVSCGFASFFFQSLCCFF	1014	PRIMARY	23

Primer_f	TACTGCATCTCCTATCACCTG
Primer_r	AAGAAACAGCAGAGAGATTGG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-