KIAA1748

Product ID	ORK00909
Accession No	AB051535
Description	zinc finger, DHHC-type containing 5
Clone name	pj02084
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4545 bp) Predicted protein sequence (758 aa)
HaloTag ORF Clone	FHC00909
Flexi ORF Clone	FXC00909
Source	Human brain (hippocampus)
Rouge ID	mKIAA1748 by Kazusa Mouse cDNA Project

Length: 4545 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1149 bp
Genome contig ID	gi51511727f_57092058
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- TTAAACCAACGAGGAATAAAAAGAAATCCTGATCT
Flanking genome sequence (133172 - 133221)	----+--------+--------+--------+--------+----* AACCAGCGCCCCCTGACTTGTGTTATTTTGTCTGGTTGAGACAGGGTGAA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	11	f	57192058	57225228	12	99.7	Perfect prediction

Length: 758 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9C0B5	100.0	Probable palmit...	Homo sapiens
Q2THX1	99.9	Probable palmit...	Pan troglodytes
Q5R838	99.7	Probable palmit...	Pongo abelii
XP_001915037	97.8	similar to memb...	Equus caballus
AAX68536	98.0	membrane-associ...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB033118	1.5e-06	37.3	KIAA1292
AB023163	5.1e-05	30.8	KIAA0946

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR001594	147	182	PD003041	Zinc finger
HMMPfam	IPR001594	138	202	PF01529	Zinc finger
ProfileScan	IPR001594	147	197	PS50216	Zinc finger

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	8	SFVLLSVASLLPFSCLFCRCVGL	30	PRIMARY	23
	2	59	PVSAAAIFLVGATTLFFAFTCPG	81	PRIMARY	23
	3	88	PAVPIYNAIMFLFVLANFSMATF	110	PRIMARY	23
	4	193	FLFLLSLTAHIMGVFGFGLLYVL	215	PRIMARY	23
	5	230	MAVMCVAGLFFIPVAGLTGFHVV	252	PRIMARY	23

Primer_f	AGTTGCAATCCATTCGTTCAG
Primer_r	GGTATAGCCTAAAGGTGGGTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-