KIAA0946

Product ID	ORK00155
Accession No	AB023163
Description	zinc finger, DHHC-type containing 17
Clone name	hj04787
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4715 bp) Predicted protein sequence (667 aa)
HaloTag ORF Clone	FHC00155
Flexi ORF Clone	FXC00155
Source	Human adult brain
Rouge ID	mKIAA0946 by Kazusa Mouse cDNA Project

Length: 4715 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2709 bp
Genome contig ID	gi89161190f_75582041
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- TTGTAAAATATTAATCAGAATAAATACTGACTCTT
Flanking genome sequence (189566 - 189615)	----+--------+--------+--------+--------+----* AAGTGTGTGCTTATGATTTCACTCAATTTACTTCAGGAAATTGATGAAAT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	12	f	75682041	75771605	17	100.0	Perfect prediction

Length: 667 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_509231	99.8	huntingtin inte...	Pan troglodytes
XP_001082309	99.7	huntingtin inte...	Macaca mulatta
Q8IUH5	100.0	Palmitoyltransf...	Homo sapiens
XP_614891	99.4	similar to hunt...	Bos taurus
BAC22089	99.7	huntingtin inte...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB051535	1.6e-10	30.6	KIAA1748
AB002377	3.2e-09	29.9	KIAA0379
AB046856	3.7e-09	29.5	KIAA1636
AB033076	5.7e-09	30.1	KIAA1250
AB095935	1.2e-08	27.1	KIAA2015

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR001594	431	504	PD003041	Zinc finger
FPrintScan	IPR002110	159	171	PR01415	Ankyrin
FPrintScan	IPR002110	272	284	PR01415	Ankyrin
HMMPfam	IPR002110	124	156	PF00023	Ankyrin
	IPR002110	158	190	PF00023	Ankyrin
	IPR002110	191	223	PF00023	Ankyrin
	IPR002110	224	257	PF00023	Ankyrin
	IPR002110	259	291	PF00023	Ankyrin
	IPR001594	468	527	PF01529	Zinc finger
HMMSmart	IPR002110	124	153	SM00248	Ankyrin
	IPR002110	158	187	SM00248	Ankyrin
	IPR002110	191	220	SM00248	Ankyrin
	IPR002110	224	254	SM00248	Ankyrin
	IPR002110	259	288	SM00248	Ankyrin
ProfileScan	IPR002110	99	312	PS50297	Ankyrin
	IPR002110	124	156	PS50088	Ankyrin
	IPR002110	158	190	PS50088	Ankyrin
	IPR002110	191	223	PS50088	Ankyrin
	IPR002110	224	257	PS50088	Ankyrin
	IPR002110	259	291	PS50088	Ankyrin
	IPR001594	472	522	PS50216	Zinc finger

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	339	KVMLGTPFLVIWLVGFIADLNID	361	PRIMARY	23
	2	363	WLIKGLMYGGVWATVQFLSKSFF	385	SECONDARY	23
	3	392	ALPLGIYLATKFWMYVTWFFWFW	414	SECONDARY	23
	4	417	LNFLFIHLPFLANSVALFYNFGK	439	SECONDARY	23
	5	515	RYFMGYLFFLLFMICWMIYGCIS	537	PRIMARY	23
	6	571	LNSVFHFMWVAVLLMCQMYQISC	593	PRIMARY	23

Primer_f	AAATGGTCAGATGGTCAGGAG
Primer_r	CCCAAAGGTTCACCATCATAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	UniGene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-