KIAA1810

Product ID	ORK00924
Accession No	AB058713
Description	synovial apoptosis inhibitor 1, synoviolin
Clone name	fk03376
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (2841 bp) Predicted protein sequence (579 aa)
HaloTag ORF Clone	FHC00924
Flexi ORF Clone	FXC00924
Source	Human fetal brain
Rouge ID	mKIAA1810 by Kazusa Mouse cDNA Project

Length: 2841 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1101 bp
Genome contig ID	gi51511727r_64551329
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- ATTGTATATACAGCCAAATAAAAACTTGCATTAAC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AATTGGTGGGGTTTGTGTCACAGAATCGATCAAATCAGTGTCGGGCTCCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	11	r	64651329	64658527	14	99.6	Perfect prediction

Length: 579 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
EAW74361	5.2e-143	100.0	synovial apopto...	Homo sapiens
AAL26903	8.4e-111	91.7	HRD1 [Homo sapi...	Homo sapiens
CAD38937	8.4e-111	91.7	hypothetical pr...	Homo sapiens
Q86TM6	2.5e-110	91.6	E3 ubiquitin-pr...	Homo sapiens
ABZ92145	3.2e-110	91.4	synovial apopto...	synthetic construct

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	NULL	302	323	PR01217	NULL
	NULL	323	339	PR01217	NULL
	NULL	348	365	PR01217	NULL
	NULL	419	444	PR01217	NULL
HMMPfam	IPR001841	254	292	PF00097	Zinc finger
HMMSmart	IPR001841	254	292	SM00184	Zinc finger
ProfileScan	IPR001841	254	293	PS50089	Zinc finger

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	16	FRTAVMMAASLALTGAVVAHAYY	38	PRIMARY	23
	2	54	SPSMAVLYIQAFVLVFLLGKVMG	76	PRIMARY	23
	3	139	DFYAILMTMVLTIFIKYVLHSVD	161	SECONDARY	23
	4	182	TGFIKVLLYMAFMTIMIKVHTFP	204	PRIMARY	23

Primer_f	CCTTCCTCTCCAACTCTTCAG
Primer_r	TGGGAACGGGAGCTAATACTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-