KIAA1973

Product ID	ORK01187
Accession No	AB075853
Description	glutamate receptor, ionotropic, N-methyl-D-aspartate 3A
Clone name	pf00154
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (7770 bp) Predicted protein sequence (1176 aa)
HaloTag ORF Clone	FHC01187
Flexi ORF Clone	FXC01187
Source	Human brain (hippocampus)
Rouge ID	mKIAA1973 by Kazusa Mouse cDNA Project

Length: 7770 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Integrity of 3' end

Length of 3'UTR	3821 bp
Genome contig ID	gi89161216r_103271456
PolyA signal sequence (AATAAA,-24)	+--------+--------+----*----+---- TATGACTGTGGAATAAATGTTCAGATAGAAACTTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACTTTTGGATGTACTGCTGGCTTTGTCTTTGGGGATTCAAATGTTGACAT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	9	r	103371456	103540683	9	99.4	Perfect prediction

Length: 1176 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001107918	97.4	similar to glut...	Macaca mulatta
BAG11375	100.0	glutamate [NMDA...	synthetic construct
Q8TCU5	99.9	Glutamate [NMDA...	Homo sapiens
EAW58958	99.7	glutamate recep...	Homo sapiens
CAC95229	99.6	N-methyl D-aspa...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB033046	0.0005	24.1	KIAA1220

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	801	870	PD245531	NULL
FPrintScan	IPR001508	654	682	PR00177	NMDA receptor
	IPR001508	738	763	PR00177	NMDA receptor
	IPR001508	805	832	PR00177	NMDA receptor
	IPR001508	992	1016	PR00177	NMDA receptor
HMMPfam	IPR001320	735	1013	PF00060	Ionotropic glutamate receptor
HMMSmart	IPR001320	626	971	SM00079	Ionotropic glutamate receptor

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	69	WLLSRVCLLLPPPCALVLAGVP	90	PRIMARY	22
	2	735	HWTMWLGIFVALHITAVFLTLY	756	PRIMARY	22
	3	773	KVFSFSSALNICYALLFGRTVAI	795	SECONDARY	23
	4	805	FLMNLWAIFCMFCLSTYTANLAA	827	SECONDARY	23
	5	990	KHFSGLFVLLCIGFGLSILTTIG	1012	PRIMARY	23

Primer_f	CAGTGCTGAAGATTATGTGAG
Primer_r	CACAGTGAGAAGTTTGCAGTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-