KIAA2013

Product ID	ORK00970
Accession No	AB095933
Description	KIAA2013
Clone name	bm04064
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector(Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGC TGAAGCTTTC ACAAGTTTGT ACAAAAAAGC AGGCTCTTC-5' end of cDNA, GAGCTCTCAC CACTTTGTAC AAGAAAGCTG GGCGGCCGCT TTTTTTTTTT TTTTTTT-3' end of cDNA).
cDNA sequence	DNA sequence (2538 bp) Predicted protein sequence (730 aa)
HaloTag ORF Clone	FHC00970
Flexi ORF Clone	FXC00970
Source	Human adult brain
Rouge ID	mKIAA2013 by Kazusa Mouse cDNA Project

Length: 2538 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	343 bp
Genome contig ID	gi89161185r_11804999
PolyA signal sequence (AATAAA,-9)	+--------+--------+----*----+---- TCAAAAAAGCAAAAAACAAAAAAGCAAATAAAAGT
Flanking genome sequence (99820 - 99771)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAAAAAATTAGAGGCAGGGCCTGGTGGCTCATGCC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	11904819	11909072	2	99.5	Perfect prediction

Length: 730 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001143176	99.7	hypothetical pr...	Pan troglodytes
XP_001143405	99.8	hypothetical pr...	Pan troglodytes
XP_001143254	99.8	hypothetical pr...	Pan troglodytes
AAI57881	96.8	LOC728138 prote...	Homo sapiens
XP_535403	94.3	similar to CG72...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	37	EARAAGPVGWAAGPAAALAPLP	58	SECONDARY	22
	2	85	LLCLLGLLLLLLWFGGSGA	103	PRIMARY	19
	3	144	EVVPLGPGVPALVANGFLALDVA	166	SECONDARY	23
	4	654	PFLFWFSVASLITLFHLFLFKLI	676	PRIMARY	23

Primer_f	AGGAAATGCTGGAGCTCTTGG
Primer_r	GCAGGAGAGCATGTAATAGAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-