KIAA1446

Gene/Protein Characteristic Table for KIAA1446

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Product ID	ORK04262
Accession No	AB040879
Description	brain-enriched guanylate kinase-associated
Clone name	fg06734
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5806 bp) Predicted protein sequence (645 aa)
Source	Human fetal brain

Length: 5806 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	816 bp
Genome contig ID	gi51511730r_99973243
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- TGTATTTTGCATTCATTAATAAAAGACACCGGTGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAGAATCTGGGTTGGGCTGTTTCTCCTCCTCCTGCCTTTTGGTCTCGGG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	14	r	100073243	100085979	5	99.0	Perfect prediction

Features of the protein sequence

Description

Length: 645 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9BUH8	1.5e-201	99.7	Brain-enriched ...	Homo sapiens
XP_547981	9.1e-187	90.1	similar to like...	Canis lupus fam...
EAW81707	6.9e-182	100.0	likely ortholog...	Homo sapiens
XP_001109589	1.2e-180	98.3	similar to like...	Macaca mulatta
AAC63268	6.4e-175	87.6	brain-enriched ...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	4	GWVCVSVCVCVCVCVR	19	PRIMARY	16

Expression profile

Description

RT-PCR-ELISA

Experimental conditions

Primer_f	GGATTCAGAGCAACTACATGG
Primer_r	TTGCAGTCCTTCCTATAGAGC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information

Description

Chromosome No. 14

Experimental conditions

Panel name	GeneBridge 4
Primer_f	TTTCTTAACGCACTTGGCCTC
Primer_r	CCTCTTCGTGGACACAACTTC
PCR product length	211 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles

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