KIAA1030

Product ID	ORK05501
Accession No	AB028953
Description	immunoglobulin superfamily, member 9B
Clone name	fh00432
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5319 bp) Predicted protein sequence (763 aa)
Source	Human fetal brain
Rouge ID	mKIAA1030 by Kazusa Mouse cDNA Project

Length: 5319 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3027 bp
Genome contig ID	gi51511727r_133190623
PolyA signal sequence (None)	+--------+--------+----*----+---- CTTCACTCCAGCCTGGGTGACAGAGATCCTGTCTC
Flanking genome sequence (99773 - 99724)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAAAAAAGAGAGAGAGAGAAGTAAGTTCCAGGCAA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	11	r	133290396	133302069	8	99.4	Perfect prediction

Length: 763 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
EAW67815	0	99.9	hCG2036598, iso...	Homo sapiens
Q9UPX0	0	99.9	Protein turtle ...	Homo sapiens
EAW67814	0	99.9	hCG2036598, iso...	Homo sapiens
XP_001087869	0	98.3	similar to immu...	Macaca mulatta
XP_001918095	1.2e-208	95.3	immunoglobulin ...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037776	4.8e-07	29.3	KIAA1355

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003961	29	112	PF00041	Fibronectin
ProfileScan	IPR003961	28	118	PS50853	Fibronectin

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	9	EVVTVNTLAFPITTPEPLVLVTP	31	SECONDARY	23
SOSUI2	2	137	VLAGIVATICFLAAAILFSTLAA	159	PRIMARY	23

Primer_f	CCTCCATCGGTGTATAATCAG
Primer_r	GCATATTTCCTTAGCTCAGCG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CCTCCATCGGTGTATAATCAG
Primer_r	GCATATTTCCTTAGCTCAGCG
PCR product length	157 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles