KIAA1215

Product ID	ORK00769
Accession No	AB033041
Description	VANGL planar cell polarity protein 2
Clone name	fh01854
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5021 bp) Predicted protein sequence (575 aa)
HaloTag ORF Clone	FHC00769
Flexi ORF Clone	FXC00769
Source	Human fetal brain
Rouge ID	mKIAA1215 by Kazusa Mouse cDNA Project

Length: 5021 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3293 bp
Genome contig ID	gi89161185f_158552090
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- TTTTTTTTATATAAAATAAAGACATTTGACTTTTG
Flanking genome sequence (112996 - 113045)	----+--------+--------+--------+--------+----* TGGGGGCATTCTGTTGCTTTTCTTTCTTTACAGGAATGGGAGCAAATGAG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	f	158652090	158665084	7	99.3	Perfect prediction

Length: 575 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_001115459	0	99.8	vang-like 2 (va...	Macaca mulatta
Q9ULK5	5.8e-207	100.0	Vang-like prote...	Homo sapiens
XP_598791	1.9e-206	99.8	similar to Ltap...	Bos taurus
Q91ZD4	5.2e-206	99.4	Vang-like prote...	Mus musculus
BAF83670	6.9e-206	99.6	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR009539	77	575	PF06638	Strabismus

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	164	AAGATLALLSFLTPLAFLLLPPL	186	PRIMARY	23
	2	196	GTACEGLFISVAFKLLILLLGSW	218	PRIMARY	23
	3	233	FVLRALLMVLVFLLVVSYWLFYG	255	PRIMARY	23
	4	272	FAVSLVDALLFVHYLAVVLLELR	294	PRIMARY	23

Primer_f	CGTACTTCTCCTCCATTGGTC
Primer_r	AATCTCACTTGGAAGGGACTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CGTACTTCTCCTCCATTGGTC
Primer_r	AATCTCACTTGGAAGGGACTC
PCR product length	198 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles