KIAA1429

Product ID	ORK00830
Accession No	AB037850
Description	KIAA1429
Clone name	fh08353
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5507 bp) Predicted protein sequence (1795 aa)
HaloTag ORF Clone	FHC00830
Flexi ORF Clone	FXC00830
Source	Human fetal brain
Rouge ID	mKIAA1429 by Kazusa Mouse cDNA Project

Length: 5507 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	118 bp
Genome contig ID	gi51511724r_95469925
PolyA signal sequence (AATAAA,-24)	+--------+--------+----*----+---- AACAAGATTTCAATAAAAATCCAAACTTTGTATGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACGTTTGTATATATTTTCCCTTTTTTGTATGACTATTTATTTAGAAAATT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	8	r	95569925	95634846	24	100.0	Perfect prediction

Length: 1795 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001089207	98.7	similar to viri...	Macaca mulatta
XP_519860	99.6	hypothetical pr...	Pan troglodytes
CAH10773	99.6	hypothetical pr...	Homo sapiens
XP_001089437	99.1	similar to viri...	Macaca mulatta
XP_001489354	98.3	similar to Prot...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	20	QSSHIDVVRFPCVVYINEVRVIP	42	SECONDARY	23
SOSUI2	2	843	SVAYNYACILILVVVQSSSDVQM	865	PRIMARY	23

Primer_f	AAAACGGCCACTCAAAGTATC
Primer_r	GCGGTAACCTGTAGAACTAAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-