KIAA1316

Product ID	ORK05408
Accession No	AB037737
Description	HEAT repeat containing 5A
Clone name	fh12964
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5477 bp) Predicted protein sequence (1590 aa)
Source	Human fetal brain
Rouge ID	mKIAA1316 by Kazusa Mouse cDNA Project

Length: 5477 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	703 bp
Genome contig ID	gi51511730r_30731838
PolyA signal sequence (None)	+--------+--------+----*----+---- CACTCCAGCCTGGGCGACAGAGTGAGACTCCATCT
Flanking genome sequence (99721 - 99672)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAATTCTGCCTTATTCAGTAATTGTATTAAACTTT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	14	r	30831559	30922706	27	99.7	Perfect prediction

Length: 1590 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAH45764	100.0	HEATR5A protein...	Homo sapiens
EAW65948	100.0	hCG41034, isofo...	Homo sapiens
NP_056288	99.9	HEAT repeat con...	Homo sapiens
Q86XA9	99.9	HEAT repeat-con...	Homo sapiens
XP_522993	99.3	hypothetical pr...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037835	2.7e-158	56.2	KIAA1414

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000357	395	431	PF02985	HEAT
HMMPfam	IPR000357	477	514	PF02985	HEAT

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	4	HPSISVRLAAAWCLHCIAVALPS	26	PRIMARY	23
	2	47	EAVTGFSFAVAALLGAVKHCPLG	69	SECONDARY	23
	3	109	ALMTLGPAVVSHHLARVLLLWKC	131	SECONDARY	23

Primer_f	CTCATGCAAATTGGACCTCAG
Primer_r	TTGACACTTTCCTGATTGCCC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CTCATGCAAATTGGACCTCAG
Primer_r	TTGACACTTTCCTGATTGCCC
PCR product length	113 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles