KIAA0447

Product ID	ORK00079
Accession No	AB007916
Description	solute carrier family 35, member E2B, transcript variant 2
Clone name	fh17910
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5272 bp) Predicted protein sequence (466 aa)
HaloTag ORF Clone	FHC00079
Flexi ORF Clone	FXC00079
Source	Human fetal brain
Rouge ID	mKIAA0447 by Kazusa Mouse cDNA Project
Note	We replaced hg00132, former representative clones for KIAA0447 with fh17910. (2005/08/06)

Length: 5272 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Integrity of 3' end

Length of 3'UTR	3613 bp
Genome contig ID	gi89161185r_1482803
PolyA signal sequence (None)	+--------+--------+----*----+---- ATGTTAAATATGGAAAAAAAAAAAAAAAAAAAAAC
Flanking genome sequence (165882 - 165833)	----+--------+--------+--------+--------+----* ACTGCTTTGTTTTCTTCCTTGTGGACCTCTGGTGTTCATGCTACTTTGTT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	1509475	1614041	11	98.7	Terminal No-hit
Ensembl gnome browser	1	r	1640910	1667316	8	98.5	Internal No-hit

Length: 466 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_001717787	2.1e-189	99.8	similar to solu...	Homo sapiens
Q5CZA4	2.9e-159	99.8	Solute carrier ...	Homo sapiens
BAG54222	6.1e-159	99.8	unnamed protein...	Homo sapiens
Q8C811	6.5e-140	86.1	Solute carrier ...	Mus musculus
XP_546710	2.5e-139	86.9	similar to solu...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR002110	84	96	PR01415	Ankyrin
FPrintScan	IPR002110	354	366	PR01415	Ankyrin
HMMPfam	IPR004853	285	430	PF03151	Protein of unknown function DUF250

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	138	LYLTLWFFFSFCTLFLNKYILSL	160	PRIMARY	23
	2	169	GAVQMLSTTVIGCVKTLVPCCLY	191	SECONDARY	23
	3	205	MTMLFVGLMRFATVVLGLVSLKN	227	SECONDARY	23
	4	259	LVNLSLIPVMGGLALCTATEISF	281	SECONDARY	23
	5	353	YNQDVVLLLLTDGVLFHLQSITA	375	SECONDARY	23
	6	401	LSVIVFGNKITSLSAVGTALVTV	423	PRIMARY	23

Primer_f
Primer_r
PCR conditions	°C sec °C sec cycles

Panel name	GeneBridge 4
Primer_f	ACTTACCACCACAGCAGAATC
Primer_r	GTTTTGCATGTGACCTATTTC
PCR product length	106 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles