KIAA1463

Product ID	ORK04776
Accession No	AB040896
Description	DIP2 disco-interacting protein 2 homolog B (Drosophila)
Clone name	fh18591s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (7334 bp) Predicted protein sequence (1166 aa)
Source	Human fetal brain
Rouge ID	mKIAA1463 by Kazusa Mouse cDNA Project
Note	We replaced fh18591, former representative clones for KIAA1463 with fh18591s1. (2003/8/28)

Length: 7334 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3833 bp
Genome contig ID	gi89161190f_49263212
PolyA signal sequence (AATAAA,-34)	+--------+--------+----*----+---- TAATAAATATTGATGCCTGATTCTGTTTAAAAAAC
Flanking genome sequence (165509 - 165558)	----+--------+--------+--------+--------+----* CTTCACACATTGGGTGGAGGCTAGGGAATTGATAGATTTTAAATTTAACT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	12	f	49363212	49428719	29	99.4	Perfect prediction

Length: 1166 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9P265	100.0	Disco-interacti...	Homo sapiens
XP_509060	99.7	DIP2 disco-inte...	Pan troglodytes
XP_534802	99.3	similar to CG70...	Canis lupus fam...
XP_001082991	99.6	similar to DISC...	Macaca mulatta
XP_001082866	99.6	similar to DISC...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB023151	0	76.9	KIAA0934
D80006	0	77.2	KIAA0184

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000873	612	1085	PF00501	AMP-dependent synthetase and ligase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	4	MFMVAFYGCLLAEVIPVPIEVPL	26	PRIMARY	23
	2	35	QIGFLLGSCGIALALTSEVCLKG	57	SECONDARY	23
	3	643	YPPGIELIAAFYGCLYAGCIPVT	665	PRIMARY	23
	4	781	SSRQIAICLDPYCGLGFALWCLC	803	SECONDARY	23
	5	1079	SIAECAVFTWTNLLVVVVELCGS	1101	PRIMARY	23
	6	1111	LVTNVVLEEHYLIVGVVVVVDPG	1133	SECONDARY	23

Primer_f	AGTAACTCTGGTAAAGCATCC
Primer_r	TAATGTGGATAGAGTAGGAGC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-