KIAA0659

Product ID	ORK00595
Accession No	AB014559
Description	diacylglycerol lipase, alpha
Clone name	fh22726
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5687 bp) Predicted protein sequence (1049 aa)
HaloTag ORF Clone	FHC00595
Flexi ORF Clone	FXC00595
Source	Human fetal brain
Rouge ID	mKIAA0659 by Kazusa Mouse cDNA Project
Note	We replaced hk01892, former representative clones for KIAA0659 with fh22726. (2002/5/10)

Length: 5687 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2447 bp
Genome contig ID	gi51511727f_61104486
PolyA signal sequence (TATAAA,-7)	+--------+--------+----*----+---- AGGAGAGACAAATTAATATAGCTTATTCTATAAAT
Flanking genome sequence (166502 - 166551)	----+--------+--------+--------+--------+----* ATATCTGTATATAAAGGTTTCTGTATATTGTATAGAGCTGTGTATAAACT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	11	f	61204486	61270986	20	99.4	Perfect prediction

Length: 1049 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9Y4D2	100.0	Sn1-specific di...	Homo sapiens
XP_001916105	98.1	similar to Sn1-...	Equus caballus
XP_601967	97.7	similar to diac...	Bos taurus
Q5YLM1	97.5	Sn1-specific di...	Rattus norvegicus
AAI48309	97.2	Diacylglycerol ...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002921	401	541	PF01764	Lipase
ScanRegExp	IPR008262	473	482	PS00120	Lipase
ScanRegExp	IPR001844	881	892	PS00296	Chaperonin Cpn60

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	32	LFLLHTTWFVILSVVLFGLVYNP	54	PRIMARY	23
	2	64	DHGRGYLGILLSCMIAEMAIIWL	86	SECONDARY	23
	3	104	VLYVRLAILVIEFIYAIVGIVWL	126	PRIMARY	23
	4	142	TLGMVVCNWVVILSVCITVLCVF	164	PRIMARY	23

Primer_f	GCATACAGAGCTTCAGCCCAG
Primer_r	TGCCCACATTAGAGGTCCCAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GCATACAGAGCTTCAGCCCAG
Primer_r	TGCCCACATTAGAGGTCCCAG
PCR product length	186 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles