KIAA1540

Product ID	ORK00859
Accession No	AB040973
Description	G protein-coupled receptor 83
Clone name	fj00652
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4109 bp) Predicted protein sequence (424 aa)
HaloTag ORF Clone	FHC00859
Flexi ORF Clone	FXC00859
Source	Human fetal brain
Rouge ID	mKIAA1540 by Kazusa Mouse cDNA Project

Length: 4109 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2832 bp
Genome contig ID	gi51511727r_93650131
PolyA signal sequence (AATAAA,-25)	+--------+--------+----*----+---- TGCTCATCTCAATAAAATTTACTTCAGGTTTGTGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACTGTGCCTCTTGGATTTGCAATTATGTCACAACTGGTAACCTTTAAAAC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	11	r	93750131	93774066	4	99.2	Perfect prediction

Length: 424 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
ABY87919	8.8e-183	100.0	G protein-coupl...	Homo sapiens
Q9NYM4	3e-182	99.8	Probable G-prot...	Homo sapiens
AAF43705	4.1e-182	99.8	orphan G-protei...	Homo sapiens
AAH67474	5.7e-182	99.5	G protein-coupl...	Homo sapiens
XP_001088588	2.7e-180	98.6	similar to G pr...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
D13626	2.5e-07	19.4	KIAA0001

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000276	74	98	PR00237	Rhodopsin-like GPCR superfamily
	IPR000611	99	111	PR01012	Neuropeptide Y receptor
	IPR000276	107	128	PR00237	Rhodopsin-like GPCR superfamily
	IPR000611	129	141	PR01012	Neuropeptide Y receptor
	IPR000611	152	167	PR01012	Neuropeptide Y receptor
	IPR000276	152	174	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	186	207	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	241	264	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	291	315	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	328	354	PR00237	Rhodopsin-like GPCR superfamily
	IPR000611	333	342	PR01012	Neuropeptide Y receptor
	IPR000611	344	357	PR01012	Neuropeptide Y receptor
HMMPfam	IPR000276	89	346	PF00001	Rhodopsin-like GPCR superfamily
ProfileScan	IPR000276	89	346	PS50262	Rhodopsin-like GPCR superfamily
ScanRegExp	IPR000276	158	174	PS00237	Rhodopsin-like GPCR superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	KMVPHLLLLCLLPLVRATEPH	21	SECONDARY	21
	2	73	ALLIVAYSFIIVFSLFGNVLVCH	95	PRIMARY	23
	3	115	AVADIMITLLNTPFTLVRFVNST	137	SECONDARY	23
	4	187	GVIYIAVIWTMATFFSLPHAICQ	209	SECONDARY	23
	5	241	DLATFILLYILPLLIISVAYARV	263	PRIMARY	23
	6	295	LMLVVVLFALCWFPLNCYVLLLS	317	PRIMARY	23

Primer_f	ATGATTCCTTCTCTCCTGCAA
Primer_r	CATTATTTAGACAACACAGCG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-