KIAA1623

Product ID	ORK01632
Accession No	AB046843
Description	anoctamin 8
Clone name	fj04650
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4150 bp) Predicted protein sequence (1236 aa)
HaloTag ORF Clone	FHC01632
Flexi ORF Clone	FXC01632
Source	Human fetal brain
Rouge ID	mKIAA1623 by Kazusa Mouse cDNA Project
Note	We replaced hk07620, former representative clones for KIAA1623 with fj04650. (2002/5/10)

Length: 4150 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	292 bp
Genome contig ID	gi42406306r_17195034
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- ACGGCAGAAATGCAATAAAAACTATATTTCAACAG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* TGCCCGAGGTCAGAGCAGTGGAAAAGGGAGTGGGGGTGGGGGAGTCTGGG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	19	r	17295034	17306638	18	99.6	Perfect prediction

Length: 1236 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9HCE9	100.0	Anoctamin-8; Tr...	Homo sapiens
XP_541955	93.8	similar to CG15...	Canis lupus fam...
AAH27735	85.4	Ano8 protein [M...	Mus musculus
XP_894573	83.8	similar to Tmem...	Mus musculus
Q6PB70	83.7	Anoctamin-8; Tr...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR007632	475	884	PF04547	Protein of unknown function DUF590

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	364	SLPLCLACLVCVFLLMLGCFQLQ	386	PRIMARY	23
	2	397	RLARFLPKVMLALLVSVSAEGY	418	SECONDARY	22
	3	441	LIIKVVLFQFVNSYLSLFYIGFY	463	SECONDARY	23
	4	760	GYVVLFSSAFPLAALCALVNNLI	782	PRIMARY	23
	5	811	KVMEAMGVLAIVVNCYLIGQCGQ	833	PRIMARY	23
	6	845	AAIVSVVVLEHFALLLKYLIHVA	867	PRIMARY	23

Primer_f	TCCTCAAGTACCTCATCCACG
Primer_r	TGGCGCTCGTGTCTCTTAAAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	RH-map
Primer_f	TTCCTCAAACTGTCCTCCCCC
Primer_r	GGGCATCCAGGCACATAAAGG
PCR product length	136 bp
PCR conditions	95 °C15 sec66 °C60 sec35 cycles